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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular flow cytometry using our FOXP3 Fix/Perm protocol. For immunofluorescent staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume or 5 µl per 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Thornton AM, et al. 2010. J. Immunol.. 184:1. PubMed 2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129. 3. Wainwright, DA., et al. 2011. Neuro Oncol. PubMed.
C57BL/6 splenocytes surface stained with CD4-PE (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-Alexa Fluor® 647 (clone 22F6).
Human peripheral blood lymphocytes surface stained with CD4-PE (clone RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-Alexa Fluor® 647 (clone 22F6).
Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation of regulatory T cells. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is involved in tumor immunity.
Structure:
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution:
Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function:
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Antigen References:
1. Kelly CM, et al. 1998. Curr. Biol. 8:508. 2. Dovat S, et al. 2005. J. Immunol. 175:3508. 3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167. 4. Cai Q, et al. 2009. J. Immunol. 183:2303. 5. Zhang Z, et al. 2007. Blood 109:2190. 6. Hahm K, et al. 1998. Genes Dev. 12:782.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse/human Helios
C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-FITC (clone 22F6).
Human peripheral blood lymphocytes surface stained with CD4-APC (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-FITC (clone 22F6).
PE anti-mouse/human Helios
C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-PE (clone 22F6).
Human peripheral blood lymphocytes surface stained with CD4-APC (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-PE (clone 22F6).
Alexa Fluor® 647 anti-mouse/human Helios
C57BL/6 splenocytes surface stained with CD4-PE (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-Alexa Fluor® 647 (clone 22F6).
Human peripheral blood lymphocytes surface stained with CD4-PE (clone RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-Alexa Fluor® 647 (clone 22F6).
Pacific Blue™ anti-mouse/human Helios
Human peripheral blood lymphocytes surface stained with CD4-PE (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-Pacific Blue™ 647 (clone 22F6).
C57BL/6 splenocytes surface stained with CD4-PE (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-Pacific Blue™ (clone 22F6).
Alexa Fluor® 488 anti-mouse/human Helios
C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-Alexa Fluor® 488 (clone 22F6).
Human peripheral blood lymphocytes surface stained with CD4-APC (clone RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-Alexa Fluor® 488 (clone 22F6).
APC anti-mouse/human Helios
Human peripheral blood lymphocytes surface stained with CD4-PE/Cy7, fixed and permeabilized with BioLegend FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-APC (clone 22F6).
C57BL/6 mouse splenocytes surface stained with CD4-PE/Cy7, fixed and permeabilized with BioLegend FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-APC (clone 22F6).
Purified anti-mouse/human Helios
C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-PE (clone 22F6).
Human peripheral blood lymphocytes surface stained with CD4-APC (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-PE (clone 22F6).
