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Monensin Solution (1,000X)
Monensin Solution (1,000X)
420701
1 ml
$45.00
Product Details
Formulation:
Monensin Solution is supplied as a 1000X working solution in 70% Ethanol. Dilute to 1X in cell culture medium.
Storage & Handling:
Store the solution at 4° C. Monensin is known to be toxic; avoid direct body contact. Ethanol is flammable; keep away from sources of fire.
Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.
COA:
Application References:
1. Smeltz RB. 2007. J. Immunol. 178:4786. 2. Fuse S, et al. 2007. J. Immunol. 178:5227. 3. Durkin ET,et al. 2008. Blood 10:1182.PubMed 4. Durkin ET, et al. 2008. Blood 112:5245. PubMed 5. Liu XS, et al. 2009. J. Immunol. 183:51. PubMed 6. Mattarollo SR, et al. 2010. J. Immunol. 184:1242. PubMed 7. Yang M, et al. 2010. J. Immuonl. 184:3321. PubMed 8. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. PubMed 9. Wood MW, et al. 2011. Infect Immun. 79:708. PubMed
Description:
Monensin is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, monensin leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Monensin Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.
Antigen References:
1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD). 2. Sander B, et al. 1991. Immunol. Rev. 119:65. 3. Sander B, et al. 1993. J. Immunol. Meth. 166:201. 4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117. 5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
