MitoSpy™ mitochrondrial localization probes are cell-permeant, fluorogenic chemical reagents used for labeling mitochondria of living cells. They can be used for live-cell imaging applications or they can be fixed to facilitate mounting the specimen with antifade for inverted microscopy or confocal imaging applications. MitoSpy™ Orange CMTMRos and MitoSpy™ Red CMXRos can be fixed and permeabilized to allow for co-staining with additional antibodies. Learn more about each of the MitoSpy™ reagents and the applications each are best-suited for.


MitoSpy™ NIR




HeLa cells were stained with 0.25µM CytoPhase™ Violet dye (red) for 60 minutes at 37°C. Then 20 nM of MitoSpy™ NIR DiIC1(5) (green) was added for an additional 30 minutes at 37°C. The Z-stack images were captured on an LSM 880 with Airyscan using a 63x Oil objective. The files were Airyscan processed and a Maximum Intensity projection was created using Zen software; image courtesy of the Biophotonics Core Facility at the Salk Institute.


MitoSpy™ Red




NIH3T3 cells were stained with 100 nM of MitoSpy™ Red CMXRos (red) for 20 minutes at 37°C, fixed with 1% PFA for ten minutes at room temperature, and permeabilized with 1X True Nuclear™ Perm Buffer for ten minutes at room temperature. Then the cells were stained with Flash Phalloidin™ NIR 647 (green) for 20 minutes at room temperature and counterstained with DAPI (blue). The image was captured with a 60x objective.



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