MitoSpy™ Mitochondrial Probes
MitoSpy™ mitochrondrial localization probes are cell-permeant, fluorogenic chemical reagents used for labeling mitochondria of living cells. They can be used for live-cell imaging applications or they can be fixed to facilitate mounting the specimen with antifade for inverted microscopy or confocal imaging applications. MitoSpy™ Orange CMTMRos and MitoSpy™ Red CMXRos can be fixed and permeabilized to allow for co-staining with additional antibodies. Learn more about each of the MitoSpy™ reagents and the applications each are best-suited for.
Prepare yourself to dye.

MitoSpy™ NIR

MitoSpy™ Red

HeLa cells were stained with 0.25µM CytoPhase™ Violet dye (red) for 60 minutes at 37°C. Then 20 nM of MitoSpy™ NIR DiIC1(5) (green) was added for an additional 30 minutes at 37°C. The Z-stack images were captured on an LSM 880 with Airyscan using a 63x Oil objective. The files were Airyscan processed and a Maximum Intensity projection was created using Zen software; image courtesy of the Biophotonics Core Facility at the Salk Institute.NIH3T3 cells were stained with 100 nM of MitoSpy™ Red CMXRos (red) for 20 minutes at 37°C, fixed with 1% PFA for ten minutes at room temperature, and permeabilized with 1X True Nuclear™ Perm Buffer for ten minutes at room temperature. Then the cells were stained with Flash Phalloidin™ NIR 647 (green) for 20 minutes at room temperature and counterstained with DAPI (blue). The image was captured with a 60x objective.
Excitation/Emission Spectra Mechanism of
Live Imaging & Fixation Compatibility Cell Health
  • MitoSpy™ Green FM has excitation/emission (ex/em) peaks at 488 nm/520 nm, common for the FITC or Alexa Fluor® 488 filter set. 
  • MitoSpy™ Orange CMTMRos has ex/em peaks of 551 nm/576 nm, common for the PE, TRITC, or Alexa Fluor® 555 filter sets. 
  • MitoSpy™ Red CMXRos has ex/em peaks at 577 nm/598 nm, common for the Texas Red® or Alexa Fluor® 594 filter set. 
  • MitoSpy™ NIR DiIC1(5) has ex/em peaks of 638 nm/658 nm, common for the Alexa Fluor® 647 or APC filter set.
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