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BioLegend
BioLegend's LEGENDScreen™ products are lyophilized, fluorophore-conjugated antibodies provided in 96-well plates for the purpose of screening cell surface molecules on your cells of interest.
Pipettes of Fury
Why choose LEGENDScreen™?
  • Large selection of specificities per screen - Human Cell Screening Kit has 332 specificities plus 10 isotype controls. Mouse Cell Screening Kit has 252 cell surface marker antibodies plus 11 isotype controls.
  • Fast and easy to follow protocol.
  • Directly conjugated antibodies at pre-titrated optimal concentrations provide reliable results.
  • Full kit with staining buffer, fixation buffer, and plate sealers.

Introducing LEGENDScreen™ Mouse Cell Screening (PE) Kit (cat # 700003).
This kit contains 252 PE-conjugated monoclonal antibodies against mouse cell surface markers, plus 11 mouse, rat, and hamster Ig isotype controls. All of the antibodies have been pre-titrated to optimum concentrations, arrayed on three 96-well plates, and lyophilized in a ready to use format. LEGENDScreen™ is the most cost-effective way to test this many directly-conjugated antibodies in a single assay.
This kit can be used to screen cell lines and primary cells (splenocytes, bone marrow-derived cells, and cells isolated from tissues, etc.). The workflow is simple and easy. Just reconstitute the antibodies, add your cells and then analyze. Cell staining is done directly in the plates, so no additional transfer of reconstituted antibody is required.

Blank wells on the plates allow for adding your own markers. This format allows users to screen cells of interest directly or co-stain with other markers labeled with different fluorochromes. Positive hits from the screening can be quickly identified based on the plate maps in the manual or online, with detailed clone information. Individual fluorochrome conjugated antibodies should be used to confirm the screening results. 

The LEGENDScreen™ Mouse Cell Screening Kit provides a convenient, easy to use, and powerful tool for immunology, stem cell, and cancer research.



Download Legendscreen PDF ManualOrder LegendscreenRequest Custom Legendscreen

LegendScreen Cover
FAQs


What is the level of variability from one experiment to the other?
If the protocol is followed closely, the variability should be minimal. The variability should be similar to single vial antibody staining.

How should the kit be stored?
The kit should be stored at 2 - 8°C upon receipt. Once opened, the plates must be reconstituted immediately. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month.

How do I request a custom LEGENDScreen™ product with only my specificities of interest?
For more info, visit: biolegend.com/custom_solutions.

What are the guarantees regarding the lyophilized plate compared to the reconstituted plate?
Lyophilized product has a guaranteed shelf life of 6 months unopened. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month. Be sure to properly seal the plates to prevent evaporation and shield the antibodies from light.

I have added my own antibody solution to the lyophilized product, will the lyophilized antibody work?
Yes, as long as the fluorophores on these antibodies are compatible and proper compensation has been applied during acquisition and analysis.

I am not going to use all the reconstituted antibody solution. Can I keep the left over for later or re-dry the solution in dark?
The antibody is in a one test per well format. There will not be any antibody left if the full test is used. Customers may decide to use less than the recommended volume per test, but this is not recommended and the performance is not guaranteed. Customers may also selectively transfer certain antibodies from the original plate to a new plate and use after reconstitution. If any antibody is not used after reconstitution, the plate can be sealed and store at 2 - 8°C for a month in the dark. Once reconstituted, re-drying is not recommended as this may result in a loss of signal.  

Can I use my existing institution discount for this new product?
Yes.

If I don’t have enough cells and use much less than 4 million/mL (~ 3 x 105 cells/well), will it still work?
This may work with lower numbers of total cells, but we recommend trying to keep higher concentrations of cells for faster analysis. Of course, how many cells are needed depends on the specific application. Successful staining has been done with 1 x 105 cells/well.

Are these plates made under sterile conditions?
The plates are not sterile. You would handle them as you would handle most typical flow cytometry staining protocols.

Can I use half or less of the plate and keep the rest for later?
Yes. Customers can use half of the plate or whatever specificities they are interested in. However, the whole plate should be reconstituted. The half plate of antibodies must be transferred to another empty plate for the staining. The remaining half must be sealed and stored at 2 - 8°C in the dark and used within a month.

For more answers,
Plate Preparation


One hour before the staining, perform the following steps to prepare the plates:
  1. Remove the lyophilized plates from the aluminum pouches.
  2. Centrifuge the plates at 600 X g for 5 minutes.
  3. Check and make sure the lyophilized cakes settled to the bottom of the plates. Keep the plates upright at all times from this point forward. Handle plates with care so that the cakes are not agitated at any time.
  4. Gently settle the plates on the bench. Hold the plate firmly and carefully remove the plate cover from one corner, one well at a time, for easy opening and preventing cross-well contamination. You may need to wiggle the cover a little while pulling back slowly.
  5. Discard the cover. Do not reseal with the original cover or apply any sealer until the lyophilized antibodies have been reconstituted.
  6. Reconstitute the lyophilized antibodies immediately, with 25 µL/well of deionized water using a multichannel pipette. To avoid crosscontamination, do not let the pipette tips touch the wells. Make sure that all the cakes are dissolved in water. If a cake is stuck on the side of the well, pipette the 25 µL water added to the well and rinse the cake down to the bottom.
  7. Seal the plates with the plate sealers provided.
  8. Wait at least 15 minutes before the staining procedure. Keep the plates in the dark.
  9.  
Note:

  • PE-conjugated antibodies are light sensitive. Try to minimize the exposure of the plates to light as much as practically possible.
  • Do not open the pouches until the day you are ready to run the experiment. Once the plates are removed from the pouches, the antibodies must be reconstituted immediately.
  • If an experiment is not performed after reconstitution, plates can be sealed and stored in the dark at 4°C for up to one month.
Preparation of Cells
  • Obtain blood or desired tissue (e.g. spleen, lymph node, thymus, bone marrow) and prepare a single cell suspension. Wash cells in 1X PBS or cell culture medium of choice, and resuspend in Cell Staining Buffer at a density between 4 × 106 and 1 × 107 cells/mL.
  • For cultured cells in suspension, spin and resuspend cells in Cell Staining Buffer at a density between 4 × 106 and 1 × 107 cells/mL.
  • For cultured adherent cells, dissociate cells using a mild enzyme (e.g. Accutase® Cell Detachment Solution, Cat. No. 423201) or non-enzymatic dissociation buffer (Cell Dissociation Buffer, enzyme-free, PBS, Cat. No. 13151-014, Life Technologies). Wash cells in 1X PBS or cell culture medium of choice, and resuspend in Cell Staining Buffer at a density between 4 × 106 and 1 × 107 cells/mL.
  • Filter the cells through a 40 µm cell strainer to remove any clumps. Keep the cells on ice before use. Approximately 30 mL of cells at a density between 4 × 106 and 1 × 107 cells/mL is needed for all four plates. Lower cell density (eg. 1.5x106 cells/mL) is possible depends on the application.
  • Optional: Reagents that block Fc receptors may be useful for reducing nonspecific immunofluorescent staining. In the absence of an effective/available blocking antibody for human Fc receptors, an alternative approach is to pre-block cells with excessive irrelevant purified Ig from the same species and isotype as the antibodies used for immunofluorescent staining.
Staining Procedure
  1. Using a multichannel pipette, add 75 µL/well of cells (~ 3 - 7.5 x 105 cells/well) to the plates as prepared above.
  2. Set up extra tubes to stain cells for flow cytometer setup and compensation, if needed.
  3. Using the multichannel pipette, gently mix the cell suspensions by pipetting up and down 2 - 3 times. Be sure to change tips between each row. Avoid creating bubbles while pipetting.
  4. Incubate for 20 - 30 minutes at 4°C in the dark.
  5. Spin the plates at 500 × g for 6 minutes to pellet cells in each well. Immediately after centrifugation, dump the supernatant into the sink by quickly inverting and flicking the plate. Gently blot the plate on a paper towel, being careful not to disturb the cell pellet.
  6. Use a multi-channel pipette to deliver 200 µL of Cell Staining Buffer to each well. Gently mix up and down to resuspend cells. Be sure to change tips between each row.
  7. Repeat step 5.
  8. To fix the cells, using a multichannel pipette, aliquot 100 µL of Fixation Buffer into each well. Gently mix up and down to resuspend cells. Be sure to change tips between each row.
  9. Incubate for 10 minutes at room temperature in the dark.
  10. Repeat steps 5-6.
  11. Repeat step 5 one more time.
  12. Resuspend cells completely in 160 µL of Cell Staining Buffer per well and analyze using a flow cytometer. We recommend analyzing 70 µL of samples and collecting at least 5,000 events, but the end user may need to determine the optimal number of events to be collected. While the first plate is being analyzed, store the other plates at 4°C in the dark.

 

Staining Procedure
Tips for Successful Staining
  • Read the entire manual carefully before the experiment.
  • Plan the experiement in advance. Designate a full day for this experiment. Do not rush any step.
  • Make sure that the flow cytometer’s autosampler is well maintained and working well before the experiment. If the flow cytometer does not have an autosampler, the samples from each well of the plates should be transferred to individual FACS tubes and read manually. In this case, the volume of the samples may need to be increased to avoid running dry.
  • Make sure that enough cells have been prepared for the staining. If there are not enough cells, you may choose to separate the staining into two separate experiments (e.g. stain two plates each time).
  • Depending on the application, the cell number needed for staining can be lowered. Successful staining has been done with 1 x 105 cells/well.
  • Make sure to prepare cells for machine set up and compensation. These cells should be treated the same way as the cells for staining in the plates.
  • Handle the plates with care. Keep the plates upright at all times and be careful not to knock the plates over.
  • Protect the plates from exposure to light as much as possible.
  • Use extra caution when opening the plate cover (see Plate Preparation for instructions). After the cover is removed, do not apply any sealers on the plates until the cakes are reconstituted. Applying plate sealers or any cover onto the plate before the cakes are reconstituted can result in cakes being dislodged and stuck to the cover or escaping from the wells.
  • Some cell surface markers are sensitive to enzymatic digestion. If adherent cells are being used for staining, a mild enzyme or non-enzymatic dissociation buffer should be used when possible.
  • Make sure cells are in a single cell suspension. DNase treatment is recommended to avoid clumps caused by dead cells.
  • Analyze only 70 µL of the 160 µL samples so that a second run can be performed if necessary.
Product Performance

The LEGENDScreen™ Human Cell Screening (PE) Kit was tested and compared with BioLegend’s cataloged single vial liquid antibody reagents. For cell staining, PBMCs were isolated using Ficoll-Paque™ Plus (GE Healthcare) and 0.25 × 106 cells were added to each well after the lyophilized antibodies were reconstituted. The cells were then stained for 20 minutes at 4 °C, washed, and fixed with Fixation Buffer. The cells were then washed, resuspended in 160 µL of Cell Staining Buffer, and analyzed using an iCyt Eclipse Flow Cytometry Analyzer.

There were no significant differences in the staining patterns or median fluorescence intensity between the lyophilized product and liquid antibodies. Below are the representative typical data obtained when comparing the LEGENDScreen™ Human Cell Screening (PE) Kit vs. Cataloged liquid antibodies.

Representative Data


Download the product manual to see more data comparisons.
Plate Maps


Below are the maps for the 4 plates provided with the LEGENDScreen™ PE Human Antibody Panel. To view the antibody in each plate, mouse over the wells. Click to find out more information on the specificity and antibody in each well.
Plate 1
Plate 2
Plate 3
Plate 4
Specificity List
Location
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
Plate 1
Blank
CD1a
CD1b
CD1c
CD1d
CD2
CD3
CD4
CD5
CD6
CD7
CD8a
CD9
CD10
CD11a
CD11b
CD11b
CD11c
CD13
CD14
CD15
CD16
CD18
CD19
CD20
CD21
CD22
CD23
CD24
CD25
CD26
CD27
CD28
CD29
CD30
CD31
CD32
CD33
CD34
CD35
CD36
CD38
CD39
CD40
CD41
CD42b
CD43
CD44
CD45
CD45RA
CD45RB
CD45RO
CD46
CD47
CD48
CD49a
CD49c
CD49d
CD49e
CD49f
CD50
CD51
CD51,CD61
CD52
CD53
CD54
CD55
CD56
CD57
CD58
CD59
CD61
CD62E
CD62L
CD62P
CD63
CD64
CD66a/c/e
CD66b
CD69
CD70
CD71
CD73
CD74
CD79b
CD80
CD81
CD82
CD83
CD84
CD85
CD85d
CD85
CD85h
CD85
CD85k
Plate 2
Blank
CD86
CD87
CD88
CD89
CD90
CD93
CD94
CD95
CD96
CD97
CD99
CD100
Please Inquire
CD102
CD103
CD104
CD105
CD106
CD107a
CD108
CD109
CD111
CD112
CD114
CD115
CD116
CD117
CD119
CD122
CD123
CD124
CD126
CD127
CD129
CD131
CD132
CD134
CD135
CD137
4-1BB Ligand
CD138
CD140a
CD140b
CD141
CD143
CD144
CD146
CD148
CD150
CD152 (CTLA-4)
CD154
CD155
CD156c
CD158
CD158b
CD158d
CD158e1
CD158f
CD161
CD162
CD163
CD164
CD165
CD166
CD167a
CD169
CD170
CD172a/b (SIRPα/β)
CD172b (SIRPβ)
CD172g (SIRPγ)
CD178
CD179a
CD179b
CD180
CD181
CD182
CD183
CD184
CD193
Please Inquire
CD196
CD197
CD200
CD200R
CD201
CD202b
CD203c
CD205
CD206
CD207
CD209
CD210
CD213α2
CD215
CD218a
Plate 3
Blank
CD220
CD221
CD226
CD229
CD231
CD235ab
CD243
CD244 (2B4)
CD245
CD252
CD253
CD254
CD255
CD257
CD258
CD261
CD262
CD263
CD266
CD267
CD268
HVEM
CD271
CD273
CD274
CD275
CD276
CD277
CD278
CD279
CD282
CD284
CD286
CD290
CD294
CD298
CD300e
CD300F
CD301
CD303
CD304
CD307e
FcRL4
CD314
CD317
CD318
CD319
CD324
CD325
CD326
CD328
CD334
CD335
CD336
CD337
CD338
CD340
CD344
CD351
CD352
CD354
CD355
CD357
CD360
β2-microglobulin
CD272
C3aR
C5L2
CCR10
CD371
CD370
CX3CR1
CXCR7
Delta Opioid Receptor
DLL1
DLL4
DR3
EGFR
erbB3
FcεRIα
Please Inquire
Galectin-9
GARP
HLA-A,B,C
HLA-A2
HLA-DQ
HLA-DR
HLA-E
HLA-G
IFN-γ R b chain
Ig light chain κ
Ig light chain λ
IgD
IgM
IL-28RA
Plate 4
Blank
Integrin α9β1
integrin β5
Integrin β7
Jagged 2
LAP
Lymphotoxin β Receptor
Mac-2
CD300c
MICA/MICB
SUSD2
SUSD2
MSC
MSC,NPC
TNAP
NKp80
Notch 1
Notch 2
Notch3
Notch 4
NPC
Podoplanin
Pre-BCR
PSMA
Siglec-10
Siglec-8
Siglec-9
SSEA-1
SSEA-3
SSEA-4
SSEA-5
TCR γ/δ
TCR Vβ13.2
TCR Vβ23
TCR Vβ8
TCR Vβ9
TCR Vδ2
Vγ9
TCR Vα24-Jα18
TCR Vα7.2
TCR α/β
CD365
CD366
Tim-4
TLT-2
TRA-1-60-R
TRA-1-81
TSLPR
IgG1, κ Isotype Ctrl
IgG2a, κ Isotype Ctrl
IgG2b, κ Isotype Ctrl
IgG3,k Isotype Ctrl
IgM, κ Isotype Ctrl
IgG1, κ Isotype Ctrl
IgG2a, κ Isotype Ctrl
IgG2b, κ Isotype Ctrl
IgM, κ Isotype Ctrl
IgG Isotype Ctrl
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Plate Preparation


One hour before the staining, perform the following steps to prepare the plates:
  1. Remove the lyophilized plates from the aluminum pouches.
  2. Centrifuge the plates at 600 X g for 5 minutes.
  3. Check and make sure the lyophilized cakes settled to the bottom of the plates. Keep the plates upright at all times from this point forward. Handle plates with care so that the cakes are not agitated at any time.
  4. Gently settle the plates on the bench. Hold the plate firmly and carefully remove the plate cover from one corner, one well at a time, for easy opening and preventing cross-well contamination. You may need to wiggle the cover a little while pulling back slowly.
  5. Discard the cover. Do not reseal with the original cover or apply any sealer until the lyophilized antibodies have been reconstituted.
  6. Reconstitute the lyophilized antibodies immediately, with 25 µL/well of deionized water using a multichannel pipette. To avoid crosscontamination, do not let the pipette tips touch the wells. Make sure that all the cakes are dissolved in water. If a cake is stuck on the side of the well, pipette the 25 µL water added to the well and rinse the cake down to the bottom.
  7. Seal the plates with the plate sealers provided.
  8. Wait at least 15 minutes before the staining procedure. Keep the plates in the dark.
  9.  
Note:

  • PE-conjugated antibodies are light sensitive. Try to minimize the exposure of the plates to light as much as practically possible.
  • Do not open the pouches until the day you are ready to run the experiment. Once the plates are removed from the pouches, the antibodies must be reconstituted immediately.
  • If an experiment is not performed after reconstitution, plates can be sealed and stored in the dark at 4°C for up to one month.
Preparation of Cells
  • Obtain desired tissue (e.g. spleen, lymph node, thymus, bone marrow) and prepare a single cell suspension. Wash cells in 1X PBS or cell culture medium of choice, and resuspend in Cell Staining Buffer at a density between 4 × 106 and 1 × 107 cells/mL.
  • For cultured cells in suspension, spin and resuspend cells in Cell Staining Buffer at a density between 4 × 106 and 1 × 107 cells/mL.
  • For cultured adherent cells, dissociate cells using a mild enzyme (e.g. Accutase® Cell Detachment Solution, Cat. No. 423201) or non-enzymatic dissociation buffer (Cell Dissociation Buffer, enzyme-free, PBS, Cat. No. 13151-014, Life Technologies). Wash cells in 1X PBS or cell culture medium of choice, and resuspend in Cell Staining Buffer at a density between 4 × 106 and 1 × 107 cells/mL.
  • Filter the cells through a 40 µm cell strainer to remove any clumps. Keep the cells on ice before use. Approximately 22.5 mL of cells at a density between 4 × 106 and 1 × 107 cells/mL is needed for all three plates. Lower cell density (e.g. 1.5 x 106 cells/mL) might be used depending on the application.
  • Optional: Optional: Reagents that block Fc receptors (TruStain fcX™ (anti-mouse CD16/32) Antibody, Cat. No. 101320, BioLegend) may be useful for reducing nonspecific immunofluorescent staining.
Staining Procedure
  1. Using a multichannel pipette, add 75 µL/well of cells (~ 3 - 7.5x 105 cells/well) to the plates as prepared above.
  2. Set up extra tubes to stain cells for flow cytometer setup and compensation, if needed.
  3. Using the multichannel pipette, gently mix the cell suspensions by pipetting up and down 2 - 3 times. Be sure to change tips between each row. Avoid creating bubbles while pipetting.
  4. Incubate for 20 - 30 minutes at 4°C in the dark.
  5. Spin the plates at 500 × g for 6 minutes to pellet cells in each well. Immediately after centrifugation, dump the supernatant into the sink by quickly inverting and flicking the plate. Gently blot the plate on a paper towel, being careful not to disturb the cell pellet.
  6. Use a multi-channel pipette to deliver 200 µL of Cell Staining Buffer to each well. Gently mix up and down to resuspend cells. Be sure to change tips between each row.
  7. Repeat step 5.
  8. To fix the cells, using a multichannel pipette, aliquot 100 µL of Fixation Buffer into each well. Gently mix up and down to resuspend cells. Be sure to change tips between each row.
  9. Incubate for 10 minutes at room temperature in the dark.
  10. Repeat steps 5-6.
  11. Repeat step 5 one more time.
  12. Resuspend cells completely in 160 µL of Cell Staining Buffer per well and analyze using a flow cytometer. We recommend analyzing 70 µL of samples and collecting at least 5,000 events, but the end user may need to determine the optimal number of events to be collected. While the first plate is being analyzed, store the other plates at 4°C in the dark.
Tips for Successful Staining
  • Read the entire manual carefully before the experiment.
  • Plan the experiement in advance. Designate a full day for this experiment. Do not rush any step.
  • Make sure that the flow cytometer's autosampler is well maintained and working well before the experiment. If the flow cytometer does not have an autosampler, the samples from each well of the plates should be transferred to individual FACS tubes and read manually. In this case, the volume of the samples may need to be increased to avoid running dry.
  • Make sure that enough cells have been prepared for the staining. If there are not enough cells, you may choose to separate the staining into two separate experiments (e.g. stain two plates each time).
  • Depending on the application, the cell number needed for staining can be decreased. Successful staining has been done with 1 x 105 cells/well.
  • Make sure to prepare cells for machine set up and compensation. These cells should be treated the same way as the cells for staining in the plates.
  • Handle the plates with care. Keep the plates upright at all times and be careful not to knock the plates over.
  • Protect the plates from exposure to light as much as possible.
  • Use extra caution when opening the plate cover (see Plate Preparation for instructions). After the cover is removed, do not apply any sealers on the plates until the cakes are reconstituted. Applying plate sealers or any cover onto the plate before the cakes are reconstituted can result in cakes being dislodged and stuck to the cover or escaping from the wells.
  • Some cell surface markers are sensitive to enzymatic digestion. If adherent cells are being used for staining, a mild enzyme or non-enzymatic dissociation buffer should be used when possible.
  • Make sure cells are in a single cell suspension. DNase treatment is recommended to avoid clumps caused by dead cells.
  • Analyze only 70 µL of the 160 µL samples so that a second run can be performed if necessary.
Product Performance

The LEGENDScreen™ Mouse Cell Screening (PE) Kit was tested and compared with BioLegend's cataloged single vial liquid antibody reagents. For cell staining, cells were isolated from spleen and lymph nodes, and 3 × 105 cells were added to each well after the lyophilized antibodies were reconstituted. The cells were then stained for 20 minutes at 4°C, washed, and fixed with Fixation Buffer. The cells were then washed, resuspended in 160 µL of Cell Staining Buffer, and analyzed using an iCyt Eclipse Flow Cytometry Analyzer.

There were no significant differences in the staining patterns or median fluorescence intensity between the lyophilized product and liquid antibodies. Below are some representative data obtained when comparing the LEGENDScreen™ Mouse Cell Screening (PE) Kit vs. cataloged liquid antibodies.

Representative Data


Download the product manual to see more data comparisons.
Plate Maps


Below are the maps for the 3 plates provided with the LEGENDScreen™ PE Mouse Antibody Panel. To view the antibody in each plate, mouse over the wells. Click to find out more information on the specificity and antibody in each well.
Plate 1
Plate 2
Plate 3
Specificity List
Location
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
Plate 1
Blank
CD1d
CD2
CD3
CD4
CD5
CD6
CD8a
CD9
CD11a
CD11b
CD11c
CD14
CD16/32
CD18
CD19
CD21,CD35
CD22
CD23
CD24
CD25
CD26
CD27
CD28
CD29
CD30
CD31
CD34
CD36
CD38
CD39
CD40
CD41
CD43
CD43
CD44
CD45
CD45R/B220
CD45RB
CD47
CD48
CD49a
CD49b
CD49b
CD49d
CD49e
CD49f
CD51
CD54
CD55
CD59a
CD61
CD62L
CD63
CD64
CD66a
CD68
CD69
CD70
CD71
CD73
CD79a
CD79b
CD80
CD81
CD83
CD84
CD86
CD88
CD90.1
CD90.2
CD93
CD94
CD96
CD98
CD103
CD105
CD106
CD107a (LAMP-1)
Mac-3
CD115
CD117
CD120a
CD120b
CD121a
CD122
CD123
CD124
CD126
CD127
CD132
CD133
CD134
CD135
CD137
4-1BB Ligand
Plate 2
Blank
CD138
CD140a
CD140b
CD144
CD146
CD147
CD150
CD152
CD153
CD154
CD155
CD157
CD160
CD169
CD178
CD179a
CD180
CD183
CD193
CD194
CD195
CD196
CD197
CD199
CD200
CD200R
CD200R3
CD201
CD202b
CD205
CD206
CD207
CD210
CD223
CD226
CD229 (Ly-9)
CD252
CD253
CD254
CD255
CD256
CD262
CD265
CD266
CD267
CD268
HVEM
CD272
CD273
CD274
CD275
CD276
CD278
CD279
CD283
TLR4 (CD284)/MD2 Complex
MAIR-V
CD309
NKG2D
CD317
CD326
CD335
CD351
CD355
GITR
Allergin-1
B7-H4
CD371
CD370
Please Inquire
CXCR7
33D1
DcTRAIL-R1
DcTRAIL-R2
CD369 (Dectin-1, CLEC7A)
DLL1
Delta-like 4
DR3
ESAM
F4/80
FcεRIα
FR4
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GARP
GITR Ligand
H-2
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IgD
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IL-21R
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Jagged 2
Plate 3
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JAML
KLRG1
LAP
LPAM-1
LT beta R
Ly108
Ly-49A
Ly-49C/F/I/H
Ly49D
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Ly49H
Ly-51
Ly-6A/E
Ly-6C
Ly-6D
Ly-6G
Ly-6G/Ly-6C
Mac-2
MAIR-IV
MD-1
NK-1.1
Notch 1
Notch 2
Notch 3
Notch 4
PD-1H
PDC-TREM
PIR-A/B
Podoplanin
RAE-1γ
RAE-1δ
Siglec H
SSEA-1
SSEA-3
TCR γ/δ
TCR Vα11
TCR Vα2
TCR Vα3.2
TCR Vα 8.3
TCR Vβ 11
TCR Vβ12
TCR Vβ13
TCR Vβ2
TCR Vβ5.1, 5.2
TCR Vβ6
TCR V beta 7
TCR Vβ8
TCR Vβ9
TCR Vγ1.1
TCR Vγ1.1 + Vγ1.2
TCR Vγ2
TCR Vγ3
TCR Vδ4
TCR β chain
TER-119
TIGIT
CD365
Tim-2
CD366
Tim-4
TLT-2
Trem-like 4
IgG Isotype Ctrl
IgG Isotype Ctrl
IgG1, κ Isotype Ctrl
IgG2a, κ Isotype Ctrl
IgG2b, κ Isotype Ctrl
IgM, κ Isotype Ctrl
IgG1, κ Isotype Ctrl
IgG2a, κ Isotype Ctrl
IgG2b, κ Isotype Ctrl
IgG2c,k Isotype Ctrl
IgM, κ Isotype Ctrl
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