IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 and secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signaling events to those observed for IL-1b. IL-33 is an inducer of Th2 cytokines such as IL-5 and IL-13.

The BioLegend LEGEND MAX™ ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) provided with 96-well strip plates that are pre-coated with a capture antibody.

This Mouse IL-33 ELISA kit is specifically designed for the accurate quantitation of mouse IL-33 from cell culture supernatant, serum, or plasma. This kit is analytically validated and provides ready-to-use reagents.