Human TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), CTX, Hemorrhagic Factor, Macrophage-Derived Cytotoxic Factor, Macrophage Cytotoxic Factor (MCF), and MCT, is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. TNF-α is secreted by macro­phages, monocytes, neutrophils, T-cells (primarily CD4+ T cells), NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing. The membrane form protein, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibro­blast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-α plays a critical role in many physiological and patho­logical conditions, including neurodegenerative diseases and cancer.

BioLegend’s LEGEND MAX™ Human TNF-α ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human TNF-α specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human TNF-α ELISA Kit is specifically designed for the accurate quantitation of Human TNF-α from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.