Human anti-mouse antibodies, also known as HAMA, are antibodies found in human serum which have the ability to bind to mouse immunoglobulin. Pre-existing HAMA activity has been detected in approximately 5-10% of normal populations, which is believed to be caused by exposure to mice or mouse agents. Therapeutic use of mouse monoclonal antibodies or products derived from mouse monoclonal antibodies are becoming the most common cause of HAMA activities in patient serum. There is an increasing need to determine the levels of HAMA in human serum samples in the therapeutic, assay development, and diagnostic areas. For example, in the therapeutic area, HAMA generated in response to injected monoclonal antibodies not only can bind and reduce the efficacy of the therapeutics, but also can cause anaphylactic complications to subsequent administration of mouse monoclonal antibodies. Therefore, it is useful for the prognosis and monitoring patients received mouse monoclonal antibody treatment. In addition, HAMA due to its nature to bind mouse IgG, tend to cause false positive results in Sandwich ELISA or false negative results in competitive immunoassays utilizing mouse monoclonal antibodies. It is important to measure serum samples for HAMA in case of unexpected immunoassay results.

BioLegend’s LEGEND MAX™ Human Anti-Mouse Ig (HAMA) ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, mouse Ig is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human Anti-Mouse Ig (HAMA) ELISA Kit is specifically designed for the accurate quantitation of Human anti-mouse Ig from serum and plasma. It is ready-to-use, accurate, and sensitive.