Human Macrophage Migration Inhibitory Factor (MIF) is a 12.5 kD, 115 amino acid, non-glycosylated polypeptide and is expressed by multiple cell types in­cluding activated T cells, macrophages, eosinophils, epithelial cells, and endo­thelial cells. MIF plays many roles in biological processes such as catalytic activity, immu­nity, endocrine regulation, signal modulation, and inflammation. MIF is expressed in malignant cells including lung, liver, breast, colon, and prostate tumors.  Recombinant MIF is a mixture of monomers, dimers, and trimers. The physiologically active forms are believed to be predominantly the dimeric and trimeric forms^{1, 2}.


BioLegend’s LEGEND MAX™ Human Active MIF ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with 96 well microtiter strip plates that are pre-coated with a capture antibody, specific for dimeric, trimeric or higher levels of MIF complex.

This kit is specifically designed for the accurate quantitation of human active MIF from cell culture supernatant, serum, plasma and other biological fluids. This kit is analytically validated, with ready-to-use reagents.

1. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
2. Meyer-Siegler K, et al. 2005. BMC Cancer 5:73.