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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunohistochemistry.?»????
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: ELISA1,2, immunoprecipitation1and Western blot1,2 from cell lysates, immunohistochemical staining1, 3 of frozen and paraffin embedded tissue sections and in whole-mount mouse embryos, in vivo blocking1, 5 of VEGF-C binding to inhibit angiogenesis, lymphangiogenesis, growth and metastasis of different tumor cells, in vitro blocking4 of VEGF-C binding to induce dispersion of endothelial cell clusters.
Application References:
1. Kubo H, et al. 2000. Blood 96:546. (IP, WB, IHC, ELISA, Block) 2. Zhang L, et al. 2010. Cell. Res. Epub ahead of print (WB, ELISA) 3. Tammela T, et al. 2010. Nature 454:656. (IHC) 4. Shimizu K, et al. 2004. Cancer Sci. 95:328. (Block) 5. Matsumura K, et al. 2003. Blood 101:1367. (Block)
6 µm frozen thymus section from a naïve C57BL/6 mouse was stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green) and DAPI (blue). Magnification 40X.
6 µm frozen lung section from an influenza-infected C57BL/6 mouse stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green), anti-CD3 (red) and DAPI (blue). Magnification 40X.
VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2nd and 3rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis.
Member of Tyr protein kinase family. Extracellular portion contains 7 immunoglobulin-like domains. Ligand binding is mediated by its domains 2 and 3. Cytoplasmic portion contains 1 protein kinase domain.
Distribution:
Expressed on vascular endothelial cells during fetal development, constitutively expressed on lymphatic endothelium in adult mice, induced in tumors with active angiogenesis.
Function:
Regulates angiogenesis and lymphangiogenesis.
Ligand Receptor:
VEGF-C and D
Interaction:
Associated with VEGFR-2 in the surface.
Antigen References:
1. Uhrin P, et al. 2010. Blood 115:3997. 2. Wang Y, et al. 2010. Nature 465:483. 3. Akobsson L, et al. 2009. Biochem. Soc. T. 37:1233. 4. Tammela T, et al. 2010. Nature 454:656. 5. Huang G and Chen L. 2008. Cancer Biother. Radio. 23:661. 6. Zhang YJ and Fang JY. 2008. J. Gastroen. Hepatol. 23:856.
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LEAF™ Purified anti-mouse VEGFR-3 (FLT-4)
6 µm frozen thymus section from a naïve C57BL/6 mouse was stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green) and DAPI (blue). Magnification 40X.
6 µm frozen lung section from an influenza-infected C57BL/6 mouse stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green), anti-CD3 (red) and DAPI (blue). Magnification 40X.
Purified anti-mouse VEGFR-3 (FLT-4)
6 µm frozen thymus section from a naïve C57BL/6 mouse was stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green) and DAPI (blue). Magnification 40X.
6 µm frozen lung section from an influenza-infected C57BL/6 mouse stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green), anti-CD3 (red) and DAPI (blue). Magnification 40X.
