Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:
FC-Quality Tested IP1, Stimulation1,2,4, Depletion3-5* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4 and in vivo depletion3-5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 107506).
Application References:
1. Bluestone, J.A., et al., 1991. Immunol. Rev. 120:5. 2. Kruisbeek, A.M., et al., 1991. In Current Protocols in Immunology p. 3.12.1 - 3.12.14. 3. Hiromatsu, K., et al., 1992. J. Exp. Med. 175:49. 4. Dieli, F., et al., 1997. Eur. J. Immunol. 27:206. 5. van der Heyde, H.C., et al., 1995. J. Immunol. 154:3985. 6. Koenecke, C. et al. 2009. Eur. J. Immunol. 39:372. PubMed
Description:
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-bearing γ/δ T cells in vitro, and to deplete peripheral TCR-bearing γ/δ T cells in vivo. Although, a recent study has demonstrated that UC7-13D5 does not deplete γ/δ T cells in vivo, but rather leads to TCR internalization and thereby generates invisible γ/δ T cells5.
Other Names:
TCR-γ/δ, γ/δ TCR
Structure:
Ig superfamily, associates with CD3 complex
Distribution:
T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function:
Antigen recognition
Ligand Receptor:
Some bacterial or tumor antigens bound MHC class I
Antigen References:
1. Skarstein, K., et al., 1995. Immunology 81: 497. 2. Harrison, L.C., et al., 1996. J. Exp. Med. 184:2167. 3. Wildner, G., et al., 1996. Eur. J. Immunol. 26: 2140. 4. Brandes, M., et al., 2005. Science 309: 264. 5. Koenecke, C. et al. 2009. Eur. J. Immunol. 39:372.
invisible
γ/δ T cells5.