Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining7,8,9 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 109214).
Application References:
1. Gascoigne, N.J. 1990. J. Biol. Chem. 265:9296. 2. Kruisbeek, A., et al., 1991. In Current Protocols in Immunology. pp. 3.12.1 3. Davenport, C., et al., 1995. J. Immunol. 155:3742. 4. Drobyski, W., et al., 1996. Blood 87:5355. 5. Kummer, U., et al., 2001. Immunol. Lett. 75:153. 6. van der Heyde, H.C., et al., 1995. J. Immunol. 154:3985. 7. Tomita, K., et al., 1999. Genes Dev. 13:1203. 8. Podd, B.S., et al., 2006. J. Immunol. 176: 6532. 9. Ponomarev, E. D., et al., 2007. J. Immunol. 178:39. 10. Chappaz, S., et al., 2007. Blood doi:10.1182/blood-2007-02-074245.
C57BL/6 CD3+ splenocytes stained
with H57-597 PE and TCRγ/δ (UC7-
13D5) FITC
Description:
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation. The H57-597 antibody does not cross-react with γ/δ TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate α/β TCR-bearing T cells.
Other Names:
TCR-β chain, TCR-β, β-TCR
Structure:
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution:
Majority of T cells and thymocytes (correlated to differentiation)
Function:
Antigen recognition, T cell activation
Ligand Receptor:
Peptide bound-MHC class I and II
Antigen References:
1. Davis, M.M., et al., 1998. Ann. Rev. Immunol. 16:523. 2. Huppa, J.B., et al., 2003. Nat. Immunol. 4:749. 3. Kubo, R., et al., 1989. J. Immunol. 142:2736.
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