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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: inhibition of the cytotoxicity1-3. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 106706).
Application References:
1. Trinité B, et al. 2000. J. Immunol. 165:4202. (Block) 2. Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914. (Block) 3. Chang CL, et al. 2007. J. Immunol. 178:6984.(Block) PubMed
Mouse Fas Ligand transfected cells stained with LEAF™ purified MFL4, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
CD178 is a 40 kD member of the TNF/NGF superfamily also known as Fas ligand, FasL, Apo-1 ligand, and CD95 ligand. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The MFL4 antibody recognizes CD178 in a wide array of mouse strains and has been reported to block CD178/CD95 induced apoptosis. The MFL4 antibody is cross-reactive with rat and mouse FasL.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse/rat CD178 (FasL)
Mouse Fas Ligand transfected L5178Y cells were stained with biotinylated CD178 (clone MFL4) or Armenian hamster IgG isotype control (open histogram), followed by Sav-PE.
LEAF™ Purified anti-mouse/rat CD178 (FasL)
Mouse Fas Ligand transfected cells stained with LEAF™ purified MFL4, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
Purified anti-mouse/rat CD178 (FasL)
Mouse Fas Ligand transfected L5178Y cells were stained with purified CD178 (clone MFL4, filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by biotinylated anti-hamster IgG and Sav-PE.
