Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:
FC-Quality Tested IP1,2, WB1,2, Activation2, Induction1, Costimulation1* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, induction of homotypic adhesion of B lymphocytes1, stimulation of B cells undergo early apoptotic events1, and promotion of T cell motility2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 104908).
Application References:
1. Maecker, H. T., et al., 2000. Hybridoma 19:15. 2. Clark, K. L., et al., 2001. J. Immunol. 167:5115. 3. Bhatnagar, S. and Schorey, J. S., 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
C57BL/6 mouse splenocytes stained with Eat-2 PE
Lou rat splenocytes stained with Eat-2 PE
Description:
CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1. CD81 is expressed on T and B cells, NK cells, dendritic cells, thymocytes, endothelial cells and fibroblasts. CD81 induces B cell adhesion via the VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8 and CD82 in T cells. The Eat-2 antibody reacts with mouse and rat CD81.
Other Names:
TAPA-1
Structure:
TM4SF tetraspanin, 26 kD
Distribution:
T and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, fibroblasts
Function:
Regulates cell growth
Antigen References:
1. Barclay, A.N., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Levy, S., et al., 1998. Annu. Rev. Immunol. 16:89. 3. Maeker, H.T., et al., 1997. FASEB J. 11:428. 4. Boismenu, R., et al., 1996. Science 271:198.
