Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1
.
Application References:
1. Streeter, P. R., et al., 1988. Nature 331:41. 2. Briskin, M. J., et al., 1993. Nature 363:461. 3. Berlin, C., et al., 1993. Cell 74:185. 4. Bargatze, R. F., et al., 1995. Immunity 3:99. 5. Tanneau, G. M., et al. 1999. J. Histochem. Cytochem. 47:1581. 6. Savinov, A. Y., et al., 2003. J. Exp. Med. 197:643. 7. Rivera-Nieves, J., et al., 2005. J. Immunol. 174:2343. 8. Burke,SD.,et al. 2007. Diabetes. 56:2919. PubMed
Description:
MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin α4β7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.
Other Names:
Mucosal addressin cell adhesion molecule-1
Structure:
Type I glycoprotein, Ig superfamily
Distribution:
HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas
Function:
Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand Receptor:
Integrin a4ß7, CD62L
Antigen References:
1. Streeter, P. R., et al., 1988. Nature 331: 41 2. Briskin, M.J., et al., 1993.Nature 363: 461. 3. Berlin, C., et al. 1993. Cell 74: 185. 4. Lehnert, K., et al., 1998. Eur. J. Immunol. 28: 3605. 5. Picarella, D., et al., 1997. J. Immunol. 158: 2099.
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