Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by ELISA assay. For neutralization of one unit/ml of IL-9, a concentration of 1-10 ug/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA Detection1,2: The biotinylated D9302C12 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with Purified D8402E8 antibody (Cat. No. 504702) as the capture antibody. Neutralization2,3: The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IL-9 bioactivity (Cat. No. 504802). Additional reported applications (for the relevant formats) include: Western blotting.
Application References:
1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. 2. Renauld, J., et al. 1995. J. Leuk. Biol. 57:353. 3. Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
Description:
IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones. The D9302C12 antibody reacts with mouse IL-9. The D9302C12 antibody can neutralize the bioactivity of natural or recombinant mouse IL-9.
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Cellular Sources:
IL-2 activated TH2 lymphocytes
Cellular Targets:
T lymphocytes, erythroid precursors
Receptors:
IL-9R
Bioactivity/Activities:
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux, V. 1992. Research Immunology 143:385. 3. Renauld, J., et al. 1993. Adv. Immunol. 54:79. 4. Yang, Y. 1992. Leuk. Lymphoma 8:441.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
