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Mouse, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)
Immunogen:
CHO expressed, recombinant mouse IL-12 p70
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) IL-12/IL-23 antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The IL-12/IL-23 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this IL-12/IL-23 antibody is quality control tested by ELISA assay. The Purified C17.8 has been tested by blocking fluorochrome conjugated C17.8 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of Purified antibody (≤ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA Detection1,2,6-9 or ELISPOT Detection: The biotinylated C17.8 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified C15.6 antibody (Cat. No. 505202/505208) as capture antibody and recombinant mouse IL-12 p40 (Cat. No. 563801) or recombinant mouse IL-12 p70 (Cat. No. 563901) as the standard. Neutralization1,2,4,5,6,10: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IL-12 bioactivity in vivo and in vitro (Cat. No. 505304).
Additional reported applications (for relevant formats of this clone) include: Western blotting3, immunoprecipitation3, and intracellular immunofluorescent staining and flow cytometric analysis.
Note: For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.
Application References:
1. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260. 2. Reichmann G, et al. 1999. J. Immunol. 163:3354. 3. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672. 4. Neucath M, et al. 1995. J. Exp. Med. 182:1281. 5. Cousens LP, et al. 1999 J. Exp. Med. 189:1315. 6. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16. 7. O'Connell PJ, et al. 2006. Blood 107:1010. 8. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. 9. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed 10. Sugimoto K, et al. 2007. Gastroenterology 133:124. PubMed
Description:
The C17.8 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C17.8 antibody can neutralize the bioactivity of natural or recombinant IL-12.
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Regulation:
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Cellular Sources:
Dendritic cells, monocytes/macrophages, B cells, T cells
Cellular Targets:
T cells, NK cells
Receptors:
IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Bioactivity/Activities:
IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. 5. Oppmann B, et al. 2000 Immunity. 13:715. 6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002 J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002 J. Immunol. 168:5448. 9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
