Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) CD70 antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The CD70 antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking of CD70-CD27 binding and inhibition of T and NK cell responses1-3 , and immunohistochemistry4 of aceton-fixed frozen sections. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 104605/104606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 104604) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 104608).
Application References:
1. Oshima H, et al. 1998. Int. Immunol. 10:517. (Block IP) 2. Akiba HH, et al. 1999. J. Immunol. 162:7058. (Block) 3. Takeda K, et al. 2000. J. Immunol. 164:1741. (Block) 4. Henke A, et al. 2000. J. Virol. 74:4284. (IHC) 5. Brown SL, et al. 2007. J. Clin. Invest. 117:258.
A20 cells stained with FR70 Biotin, followed by Sav-PE
CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.
Other Names:
Ki-24, CD27-ligand, TNFSF7
Structure:
TNF/NGF superfamily, 30-33 kD
Distribution:
Activated B cells and some activated T cells
Function:
T-B cell cross-stimulation
Ligand Receptor:
CD27
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Tesselaar K, et al. 1997. J. Immunol. 159:4959. 3. Akiba H, et al. 1999. J. Immunol. 162:7058.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD70
A20 cells stained with FR70 Biotin, followed by Sav-PE
PE anti-mouse CD70
Mouse B cell line A20 stained with FR70 PE
Purified anti-mouse CD70
Mouse B cell line A20 stained with FR70 PE
LEAF™ Purified anti-mouse CD70
A20 cells stained with FR70 Biotin, followed by Sav-PE
