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Mouse, Cross-Reactivity: Not cross-reactive with human CD47
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Wang H, et al. 2007. P. Natl. Acad. Sci. USA 104:13744. 2. Jiang P, et al. 1999. J. Biol. Chem. 274:559. 3. Chang HP, et al. 2001. Neuroscience. 102:289.
Description:
CD47, also known as Integrin-Associated Protein (IAP) ,is a membrane protein of about 50 kd with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region.It is widely expressed on many cell types and often associated with beta 3 integrins.The significance of this molecules is recently drawing increasing attention.It has been reported that CD47 functions as a self marker.Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages.By binding to SIRPalpha,CD47 controls hemostatic innate immune functions,such as phagocytosis and cell trafficking.
Other Names:
Integrin-associated protein, IAP, neurophilin,gp42, Rh-associated protein
Structure:
50kd plasma membrane protein of immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids and a short cytoplasmic tail of 30 amino acids.
Distribution:
Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.
Function:
Adhesion molecules, immune regulation
Ligand Receptor:
SIRPalpha (CD172alpha, Signal regulatory protein alpha), thrombospondin
Interaction:
Associate with beta3 integrins
Bioactivity/Activities:
Upon interaction with SIRPalpha, controls innate immune effector function, such as host cell phagocytosis
Antigen References:
1. Brown E, et al. 1990. J. Cell Biol. 111:2785 2. Mawby WJ, et al. 1994. Biochem. J. 304:525 3. Gao AG, et al. 1996. J. Biol. Chem. 271:21 4. Oldenborg PA, et al. 2000. 288:2051. 5. van Beck EM, et al. 2005. J. Immunol. 175:7781
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
