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LEAF™ Purified anti-mouse CD45R/B220 Antibody
LEAF™ Purified anti-mouse CD45R/B220 Antibody
103216 500 µg $225.00     

Product Details

Clone: RA3-6B2
Isotype: Rat IgG2a, κ
Isotype Control:LEAF™ Purified Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse, Cross-Reactivity: Human, Cat (Feline)
Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells
Formulation: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:

FC - Quality tested
IP, Activ, IHC - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Application
Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103216).

Application
References:

1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
10. Lang GL, et al. 2008. Blood 111:2158. PubMed
11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed

BALB/c mouse splenocytes stained with
BALB/c mouse splenocytes stained with LEAF™ purified RA3-6B2, followed by anti-rat IgG FITC


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Description:

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Other Names: B220
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: B cells, T cell subset, NK cell subset
Function: Phosphatase, T and B cell activation
Ligand Receptor: Galectin-1, CD2, CD3, CD4
Antigen
References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

GeneID: 19264
Latest Publications: View the latest CD45R articles on HighwirePress.com
UniProt: View information about CD45R on UniProt.org
Keywords: LEAF™ Purified anti-mouse CD45R/B220, RA3-6B2, LEAF™, B220, Mouse, Cross-Reactivity: Human, Cat (Feline), Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 APC

  • Biotin anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with biotinylated RA3-6B2, followed by Sav-PE

  • FITC anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 FITC

  • LEAF™ Purified anti-mouse CD45R/B220
    BALB/c mouse splenocytes stained with

    BALB/c mouse splenocytes stained with LEAF™ purified RA3-6B2, followed by anti-rat IgG FITC

  • PE anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 PE

  • PE/Cy5 anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 PE/CY5

  • Purified anti-mouse CD45R/B220
    C57BL/6 mouse slenocytes were stained

    C57BL/6 mouse slenocytes were stained with purified B220 (clone RA3-6B2) (filled histogram) or rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.

  • PE/Cy7 anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 PE/CY7

  • APC/Cy7 anti-mouse CD45R/B220
    C57BL/6 splenocytes stained with RA3-6B2

    C57BL/6 splenocytes stained with RA3-6B2 APC/Cy7

  • Alexa Fluor® 488 anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 647

  • Pacific Blue™ anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with Pacific Blue™ RA3-6B2

  • Alexa Fluor® 700 anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 700

  • PerCP anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 PerCP

  • PerCP/Cy5.5 anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RA3-6B2 PerCP/Cy5.5

  • Brilliant Violet 421™ anti-mouse CD45R/B220
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD45R/B220 (clone RA3-6B2) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).

  • Brilliant Violet 570™ anti-mouse CD45R/B220
    57BL/6 mouse splenocytes were stained

    57BL/6 mouse splenocytes were stained with CD3 FITC and CD45R/B220 (clone RA3-6B2) Brilliant Violet 570™ (top) or rat IgG2a, κ Brilliant Violet 570™ isotype control (bottom).





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