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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103216).
Application References:
1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP) 2. George A, et al. 1994. J. Immunol. 152:1014. (Activ) 3. Asensi V, et al. 1989. Immunology 68:204. (Activ) 4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ) 5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) 6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC) 7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC) 8. Chang C L-T, et al. 2007. J. Immunol. 178:6984. 9. Fazilleau N, et al. 2007. Nature Immunol. 8:753. 10. Lang GL, et al. 2008. Blood 111:2158. PubMed 11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
BALB/c mouse splenocytes stained with LEAF™ purified RA3-6B2, followed by anti-rat IgG FITC
CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.
Other Names:
B220
Structure:
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution:
B cells, T cell subset, NK cell subset
Function:
Phosphatase, T and B cell activation
Ligand Receptor:
Galectin-1, CD2, CD3, CD4
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 APC
Biotin anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with biotinylated RA3-6B2, followed by Sav-PE
FITC anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 FITC
LEAF™ Purified anti-mouse CD45R/B220
BALB/c mouse splenocytes stained with LEAF™ purified RA3-6B2, followed by anti-rat IgG FITC
PE anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 PE
PE/Cy5 anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 PE/CY5
Purified anti-mouse CD45R/B220
C57BL/6 mouse slenocytes were stained with purified B220 (clone RA3-6B2) (filled histogram) or rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
PE/Cy7 anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 PE/CY7
APC/Cy7 anti-mouse CD45R/B220
C57BL/6 splenocytes stained with RA3-6B2 APC/Cy7
Alexa Fluor® 488 anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with Pacific Blue™ RA3-6B2
Alexa Fluor® 700 anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 700
PerCP anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 PerCP
PerCP/Cy5.5 anti-mouse CD45R/B220
C57BL/6 mouse splenocytes stained with RA3-6B2 PerCP/Cy5.5
Brilliant Violet 421™ anti-mouse CD45R/B220
C57BL/6 mouse splenocytes were stained with CD45R/B220 (clone RA3-6B2) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-mouse CD45R/B220
57BL/6 mouse splenocytes were stained with CD3 FITC and CD45R/B220 (clone RA3-6B2) Brilliant Violet 570™ (top) or rat IgG2a, κ Brilliant Violet 570™ isotype control (bottom).
