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LEAF™ Purified Anti-mouse CD4 Antibody
Material Safety Data Sheet (MSDS) LEAF <Low Endotoxin Azide-Free>Purified Anti-mouse CD4 Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
LEAF™ Purified Anti-mouse CD4 Antibody
100415 50 µg $55.00     
100416 500 µg $195.00     
100435 1 mg $360.00     
Clone: GK1.5
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse CTL clone V4
Formulation: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:

FC - Quality tested
Blocking1,4,11*, Costimulation3*, Depletion2,5-8*, IHC(Frozen)9,10*, IP1,2* - *This application has been reported in the literature.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application
Notes:

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-cell to sperm cell adhesion1,4, immunohistochemical staining9,10 of acetone-fixed frozen sections, and immunoprecipitation1,2. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody (Cat. No. 100506), but not RM4-4 antibody (Cat. No. 116002). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100416).

Application
References:

1. Dialynas, D.P., et al., 1983. J. Immunol. 131:2445.
2. Dialynas, D.P., et al., 1983. Immunol. Rev. 74:29.
3. Wu, L., et al., 1991. J. Exp. Med. 174:1617.
4. Godfrey, D.I., et al., 1994. J. Immunol. 152:4783.
5. Gavett, S.H., et al., 1994. Am. J. Respir. Cell. Mol. Biol. 10:587.
6. Schuyler, M., et al., 1994. Am. J. Respir. Crit. Care Med. 149:1286.
7. Ghobrial, R.R., et al., 1989. Clin. Immunol. Immunopathol. 52:486.
8. Israelski, D.M., et al., 1989. J. Immunol. 142:954.
9. Zheng, B., et al., 1996. J. Exp. Med. 184:1083.
10. Frei, K., et al., 1997. J. Exp. Med. 185:2177.
11. Felix, N.J., et al., 2007. Nature Immunol. 8:388.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with LEAF™ purified GK1.5, followed by anti-rat IgG FITC


Description:

CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. It is also useful for in vivo and in vitro depletion of CD4+ T cells.

Other Names: L3T4, T4
Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, T cell subset
Function: TCR co-receptor, T cell activation
Ligand Receptor: MHC class II molecule
Antigen
References:

1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Bierer, B.E., et al., 1989. Annu. Rev. Immunol. 7:579.
3. Janeway, C.A. 1992. Annu. Rev. Immunol. 10:645.

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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