H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:
FC - Quality tested IHC(Fr/P)5,14,15*, IP1*, WB4*, C'6*, Inhib1,2,7,12*, Apop3*, Depl8-10*, Activ1,16*, IF5* - *Reported in literature
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletions8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific of T cells11. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100314).
Application References:
1. Leo, O., et al., 1987. PNAS 84:1374. 2. Kruisbeek, A.M., et al., 1991. In Current Protocols in Immunology. pp. 3.12.1 - 3.12.14. 3. Duke, R.C., et al., 1995. Current Protocols in Immunology. pp. 3.17.1 - 3.17.33. 4. Salvadori, S., et al., 1994. J. Immunol. 153:5176. 5. Payer, E., et al., 1991. J. Immunol. 146:2536. 6. Jacobs, H., et al., 1994. Eur. J. Immunol. 24:934. 7. Vossen, A.C.T.M., et al., 1995. Eur. J. Immunol. 25:1492. 8. Henrickson, M. et al., 1995. Transplantation 60:828. 9. Kinnaert, P., et al., 1996. Transpl. Int. 9:386. 10. Han, W.R., et al., 1999. Transpl. Immunol. 7:207. 11. Miescher, G.C., et al., 1989. Immunol. Lett. 23:113. 12. Terrazas, L. I., et al., 2005. Intl. J. Parasitology. 35:1349. 13. Ko, S-Y., et al., 2005. J. Immunol. 175:3309. 14. Podd, B.S., et al., 2006. J. Immunol. 176: 6532. 15. Tilley, S.L., et al., 2007. J. Immunol. 178:3208. 16. Wang, W., et al., 2007. J. Immunol. 178:4885. 17. Xiao, S., et al., 2007. J. Exp. Med. 204:1691.
C57BL/6 mouse splenocytes stained with LEAF™ purified 145-2C11, followed by anti-Armenian hamster IgG FITC
Description:
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions.
Other Names:
CD3ε, T3, CD3
Structure:
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ? subunits and TCR (α/β and γ/δ), 20 kD
Distribution:
Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function:
TCR signal transduction, T cell activation, antigen recognition
Ligand Receptor:
Peptide antigen/MHC-complex
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Davis, M.M. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss, A., et al., 1994. Cell 76:263.
