H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) CD3ε antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The CD3ε antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this CD3ε antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining5,14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletions8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific of T cells11. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100314).
Application References:
1. Leo, O., et al., 1987. PNAS 84:1374. 2. Kruisbeek, A.M., et al., 1991. In Current Protocols in Immunology. pp. 3.12.1 - 3.12.14. 3. Duke, R.C., et al., 1995. Current Protocols in Immunology. pp. 3.17.1 - 3.17.33. 4. Salvadori, S., et al., 1994. J. Immunol. 153:5176. 5. Payer, E., et al., 1991. J. Immunol. 146:2536. 6. Jacobs, H., et al., 1994. Eur. J. Immunol. 24:934. 7. Vossen, A.C.T.M., et al., 1995. Eur. J. Immunol. 25:1492. 8. Henrickson, M. et al., 1995. Transplantation 60:828. 9. Kinnaert, P., et al., 1996. Transpl. Int. 9:386. 10. Han, W.R., et al., 1999. Transpl. Immunol. 7:207. 11. Miescher, G.C., et al., 1989. Immunol. Lett. 23:113. 12. Terrazas, L. I., et al., 2005. Intl. J. Parasitology. 35:1349. 13. Ko, S-Y., et al., 2005. J. Immunol. 175:3309. 14. Podd, B.S., et al., 2006. J. Immunol. 176: 6532. 15. Tilley, S.L., et al., 2007. J. Immunol. 178:3208. 16. Wang, W., et al., 2007. J. Immunol. 178:4885. 17. Xiao, S., et al., 2007. J. Exp. Med. 204:1691. 18. Chappaz, S., et al., 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed. 19. Curtsinger,JM.,et al.2005. J Immunol175:4392. PubMed 20. Guo, Y., et al. 2008.Blood. 112:480. PubMed 21. Kenna, TJ., et al. 2008. Blood. 111:2091 22. Perchonock, CE., et al. 2007. J Immunol. 179:1768. PubMed 23. Perchonock, GE.,et al. 2006. Mol Cell Biol. 26:6005. PubMed 24. Kanaya T. et al.,2008.Am J Physiol Gastrointest Liver PhysiolPubMed 25. de Koning,BA.,et al.2006. Int Immunol.18:941.PubMed 26. Schulteis,RD.,et al. 2008. Blood. 295:G273. PubMed 27. Qi, Q., et al. 2009. Blood. 114:564. PubMed
C57BL/6 mouse splenocytes stained with LEAF™ purified 145-2C11, followed by anti-Armenian hamster IgG FITC
Description:
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions.
Other Names:
CD3ε, T3, CD3
Structure:
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ? subunits and TCR (α/β and γ/δ), 20 kD
Distribution:
Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function:
TCR signal transduction, T cell activation, antigen recognition
Ligand Receptor:
Peptide antigen/MHC-complex
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Davis, M.M. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss, A., et al., 1994. Cell 76:263.
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