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Recombinant mouse GITR protein and Transfected cells
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 120216).
Application References:
1. Cobbold SP, et al. 2004. J. Immunol. 172:6003. 2. Biburger M, et al. 2008. J. Leukocyte Biol. 84:264. PubMed
Description:
GITR, Glucocorticoid-induced TNFR-related gene, is a member of the TNF receptor superfamily, also known as TNFRSF18, and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD4+CD25+ T regulatory (Treg) cells. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation, and abrogate the inhibitory functions of CD4+CD25+ Treg cells. In vivo activation GITR causes development of autoimmune diseases and restores the suppressed immune responses.
Other Names:
TNFRSF18, AITR
Structure:
TNF receptor superfamily member
Distribution:
Low levels on resting T cells, high on CD4+CD25+ Treg cells, expression upregulated upon activation.
Function:
Co-stimulate GITR expressing cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand Receptor:
GITR ligand (TNFSF18)
Antigen References:
1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059 2. Ronchetti S, et al. 2004. Eur. J. Immunol. 34:613 3. Kanamaru F, et al. 2004. J. Immunol. 172:613 4. Shimizu J, et al. 2002. Nat. Immunol. 3:135 5. Stephens GL, et al. 2004. J. Immunol. 173:5008 6. McHugh RS, et al. 2002. Immunity 16:311
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
