Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
This antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:
FC, FA
Recommended Usage:
Each lot of this antibody is quality tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 μg per 106 cells in 100 μl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
Maier H., et al., 2007. J Immunol. 178:2714.
C57BL/6 splenocytes stained with LEAF™ purified 10F.9G2, followed by biotinylated anti-rat IgG and Sav-PE
Description:
CD274, also known as B7-H1 or programmed death ligand (PDL1), is a 40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production.
Other Names:
B7-H1, PD-L1
Structure:
a 40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution:
T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
Ligand Receptor:
PD-1 (PDCD1)
Bioactivity/Activities:
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Its interaction with PDCD1 inhibit T-cell proliferation and cytokine production.
