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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 109308).
Application References:
1. Kayagaki N, et al. 1999. J. Immunol. 163:1906. (Block) 2. Sato K, et al. 2005. J. Immunol. 174:4025. (Block) 3. Joshi PS, et al. 2006. J. Leukocyte Biol. 80:1345. 4. Herold S, et al. 2008. J. Exp. Med. 205:3065. PubMed 5. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed 6. Komatsu M, et al. 2003. Blood 101:3991. (Block) 7. Taieb J, et al. 2006. Nature Med. 12:214. (Block)
Mouse TRAIL transfected cells stained with LEAF™ purified N2B2, followed by anti-rat IgG FITC
CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicity in vitro.
TNF ligand superfamily member, approximately 40 kD
Distribution:
Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells
Function:
Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity
Ligand Receptor:
TRAIL receptor DR5
Antigen References:
1. Kayagaki N, et al. 1999. J. Immunol. 163:1906. 2. Wiley SR, et al. 1995. Immunity 3:673. 3. Wu GS, et al. 1999. Cancer Res. 59:2770. 4. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected cells stained with purified N2B2, followed by anti-rat IgG FITC
Biotin anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected cells stained with biotinylated N2B2, followed by Sav-PE
PE anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected cells stained with N2B2 PE
LEAF™ Purified anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected cells stained with LEAF™ purified N2B2, followed by anti-rat IgG FITC
