Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:
FC - Quality tested IP1,2*, WB1*, Blocking2-6*, Stimulation2-6*, IHC(Frozen/ Paraffin)7* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101410).
Application References:
1. Sanchez-Madrid, et al., 1983. J. Exp. Med. 158:586. 2. Zahalka, M.A., et al., 1993. J. Immunol. 150:4466. 3. Zahalka, M.A., et al., 1994. Int. Immunol. 6:917. 4. Ishida, Y., et al., 1994. Cell. Immunol. 155:414. 5. Isobe, M., et al., 1992. Science 255:1125. 6. Driessens, M.H.E., et al., 1996. J. Leukoc. Biol. 60:758. 7. Barlow, S. C., et al., 2004. Amer. J. Pathol. 165:1849.
C57BL/6 mouse splenocytes stained with LEAF™ purified M18/2, followed by anti-rat IgG FITC
Description:
CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.
Other Names:
integrin β2, β2 integrin
Structure:
Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
