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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The LEAF™Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101410).
Application References:
1. Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB) 2. Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim) 3. Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim) 4. Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim) 5. Isobe M, et al. 1992. Science 255:1125. (Block, Stim) 6. Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim) 7. Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)
C57BL/6 mouse splenocytes stained with LEAF™ purified M18/2, followed by anti-rat IgG FITC
CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.
Other Names:
integrin β2, β2 integrin
Structure:
Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175. 3. Wilson RW, et al. 1993. J. Immunol. 151:1571.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD18
C57BL/6 mouse splenocytes stained with M18/2 FITC
LEAF™ Purified anti-mouse CD18
C57BL/6 mouse splenocytes stained with LEAF™ purified M18/2, followed by anti-rat IgG FITC
PE anti-mouse CD18
C57BL/6 splenocytes stained with
M18/2 PE
Purified anti-mouse CD18
C57BL/6 mouse splenocytes stained with purified M18/2, followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD18
C57BL/6 mouse splenocytes stained with M18/2 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD18
C57BL/6 mouse splenocytes stained with M18/2 Alexa Fluor® 647
