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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for relevant formats) include: immunoprecipitation1, Western blotting1, immunocytochemistry staining1, immunohistochemical staining of frozen tissue sections1 and blocking cell interactions in vivo1.
Application References:
1. Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (IP WB IF IHC Block) 2. Liao F, et al. 2000. Cancer Res. 60:6805. (FC) 3. Crosby CV, et al. 2005. Blood 105:2771. (FC)
Mouse endothelial cells b.End.3 stained with purified BV13 conjugated with Alexa Fluor® 647
CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family. It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion. VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.
Other Names:
Vascular endothelial-cadherin
Structure:
A 120 kD member of type II Cadherin family
Distribution:
Endothelial cells
Function:
Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion.
Ligand Receptor:
CD144
Antigen References:
1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821. 2. Hirashima M, et al. 2009. Blood 93:1253. 3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. 4. Matsumura K, et al. 2003. Blood 101:1367. 5. Hirashima M, et al. 2009. Blood 101:2261. 6. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD144 (VE-cadherin)
Mouse endothelial cells b.End.3 stained with purified BV13 conjugated with Alexa Fluor® 647
LEAF™ Purified anti-mouse CD144 (VE-cadherin)
Mouse endothelial cells b.End.3 stained with purified BV13 conjugated with Alexa Fluor® 647
Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin)
Mouse endothelial cells b.End.3 stained with BV13 Alexa Fluor® 647
Biotin anti-mouse CD144 (VE-cadherin)
Mouse endothelial cells bEnd.3 stained with biotinylated anti-mouse CD144 (clone BV13), followed by Sav-PE
PE anti-mouse CD144 (VE-cadherin)
Mouse endothelial cells bEnd.3 stained with PE anti-mouse CD144 (BV13 PE)
APC anti-mouse CD144 (VE-cadherin)
bEnd.3 cells surface stained with CD144 (clone BV13) APC (filled histogram) or rat IgG1 APC isotype control (open histogram).
