Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:
FC6-9 - Quality tested IP1*, Blocking2*, IHC(Frozen)3-5*, Depletion11-12* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1, in vitro blocking of cell-cell adhesion2, and immunohistochemistry3-5 of acetone-fixed frozen
Application References:
1. Springer, T., et al., 1978. Eur. J. Immunol. 8:539. 2. Springer, T.A., et al., 1982. Immunol. Rev. 68:171. 3. Flotte, T.J., et al., 1983. Am. J. Pathol. 111:112. 4. Hata, H., et al., 2004. J. Clin. Invest. 114:582. 5. Zhang, Y., et al., 2002. J. Immunol. 168: 3088. 6. Olver, S., et al., 2006. Cancer Research 66:571. 7. Tan, S-L., et al., 2006. J. Immunol. 176:2872. 8. Ponomarev, E. D., et al., 2006. J. Immunol. 176:1402. 9. Dzhagalov, I., et al 2007. Blood 109:1620. 10. Fazilleau, N., et al., 2007. Nature Immunol. 8:753. 11. Ault, K., Springer, T., 1981. J. Immunol. 126:359. . 12. D'Amico, A., Wu, L., 2003. J. Exp. Med. 198:293.
C57BL/6 mouse bone marrow cells stained with LEAF™ purified M1/70, followed by anti-rat IgGs FITC
Description:
CD11b is a 170 kD glycoprotein, also known as αM integrin, Mac-1 α subunit, Mol, CR3, or Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, NK cells, and some T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. The M1/70 antibody can block CD11b-mediated cellular adhesion. This antibody is also cross-reactive with human CD11b.
Other Names:
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR
Structure:
Integrin family, associates with integrin ß2 (CD18), 170 kD
Distribution:
Granulocytes, monocytes, NK cells, some T and B cells
