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LEAF™ Purified Anti-mouse CD11b Antibody
Material Safety Data Sheet (MSDS) LEAF Purified Anti-mouse CD11b<NA/LE Azide Free NALE> Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
LEAF™ Purified Anti-mouse CD11b Antibody
101213 50 µg $70.00     
101214 500 µg $240.00     
101231 1 mg $450.00     
Clone: M1/70
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Cross-Reactivity: Human, Cynomolgus, Rhesus, Rabbit (Lapine)
Immunogen: C57BL/10 splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC6-9 - Quality tested
IP1*, Blocking2*, IHC(Frozen)3-5*, Depletion11-12* - *This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1, in vitro blocking of cell-cell adhesion2, and immunohistochemistry3-5 of acetone-fixed frozen
Application
References:
1. Springer, T., et al., 1978. Eur. J. Immunol. 8:539.
2. Springer, T.A., et al., 1982. Immunol. Rev. 68:171.
3. Flotte, T.J., et al., 1983. Am. J. Pathol. 111:112.
4. Hata, H., et al., 2004. J. Clin. Invest. 114:582.
5. Zhang, Y., et al., 2002. J. Immunol. 168: 3088.
6. Olver, S., et al., 2006. Cancer Research 66:571.
7. Tan, S-L., et al., 2006. J. Immunol. 176:2872.
8. Ponomarev, E. D., et al., 2006. J. Immunol. 176:1402.
9. Dzhagalov, I., et al 2007. Blood 109:1620.
10. Fazilleau, N., et al., 2007. Nature Immunol. 8:753.
11. Ault, K., Springer, T., 1981. J. Immunol. 126:359. .
12. D'Amico, A., Wu, L., 2003. J. Exp. Med. 198:293.
C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with LEAF™ purified M1/70, followed by anti-rat IgGs FITC


Description: CD11b is a 170 kD glycoprotein, also known as αM integrin, Mac-1 α subunit, Mol, CR3, or Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, NK cells, and some T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. The M1/70 antibody can block CD11b-mediated cellular adhesion. This antibody is also cross-reactive with human CD11b.
Other Names: αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR
Structure: Integrin family, associates with integrin ß2 (CD18), 170 kD
Distribution: Granulocytes, monocytes, NK cells, some T and B cells
Function: Adhesion, chemotaxis
Ligand Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Antigen
References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Springer, T.A. 1994. Cell 76:301.
3. Coxon, A., et al., 1996. Immunity 5:653.
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LEAF™ Purified Rat IgG2b, κ Isotype CtrlRTK4530FA,FC,ICC,ICFC,IF,IHC,IP,WB