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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
This clone is reported to have some effect on increasing LFA-1-mediated adhesion and does not block LFA-1 binding1.
Application References:
1. Pont S, et al. 1986. J. Immunol. 136:3750. (FC)
C57BL/6 splenocytes were stained with LFA-1 (clone H155-78) PerCP/Cy5.5 (filled histogram) or rat IgG1 PerCP/Cy5.5 isotype control (open histogram).
Lymphocyte function-associated antigen 1 or LFA-1 is a heterodimer composed of two members of the integrin family, αL (CD11a) and β2 (CD18). LFA-1 is expressed primarily on lymphocytes, monocytes/macrophages, and granulocytes. It is involved in cell adhesion and costimulation processes. The ligands for LFA-1 are ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
Other Names:
Lymphocyte function-associated antigen 1
Structure:
Heterodimer composed of two members of the integrin family, &alphaL (CD11a) and β2 (CD18).
Distribution:
Primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes
Function:
Intercellular adhesion and costimulation
Ligand Receptor:
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), and ICAM-3 (CD242)
Antigen References:
1. Dugger KJ, et al. 2009. J. Neuroimmunol. 206:22. 2. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press. 3. Springer TA. 1994. Cell 76:301. 4. Lub M, et al. 1995. Immunol. Today 16:479.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
LEAF™ Purified anti-mouse CD11a/CD18 (LFA-1)
C57BL/6 splenocytes were stained with LFA-1 (clone H155-78) PerCP/Cy5.5 (filled histogram) or rat IgG1 PerCP/Cy5.5 isotype control (open histogram).
PerCP/Cy5.5 anti-mouse CD11a/CD18 (LFA-1)
C57BL/6 splenocytes were stained with LFA-1 (clone H155-78) PerCP/Cy5.5 (filled histogram) or rat IgG1 PerCP/Cy5.5 isotype control (open histogram).
Purified anti-mouse CD11a/CD18 (LFA-1)
C57BL/6 splenocytes were stained with LFA-1 (clone H155-78) PerCP/Cy5.5 (filled histogram) or rat IgG1 PerCP/Cy5.5 isotype control (open histogram).
PE anti-mouse CD11a/CD18 (LFA-1)
C57BL/6 mouse splenocytes were stained with H155-78 PE and rat IgG1 PE isotype control.
