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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining4 of acetone-fixed frozen sections, immunofluorescence microscopy6, and in vitro blocking of LPS-induced cytokine production2,3,7,9. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 312807).
Application References:
1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777. 2. Wang R, et al. 2003. Hybrid Hybridomics 22:357. (Block) 3. Wang JE, et al. 2001. Infect. Immun. 69:2402. (Block) 4. Ishihara S, et al. 2004 J. Immunol. 173:1406. (IHC) 5. Kawahara T, et al. 2001 Infect. Immun. 69:4382. 6. Jiang Q, et al. 2000. J. Immunol. 165:3541. (IF) 7. Sugawara S, et al. 2001. Infect. Immun. 69:4951. (Block) 8. Chavakis E, et al. 2007. Circ. Res. 100:204. PubMed 9. Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149. (Block) PubMed
Human peripheral blood monocytes stained with HTA125 PE
Toll-like receptors are type I transmembrane signaling receptors. They are primordial pathogen-recognition proteins that function as sentinels for the innate immune system. TLR4, also known as CD284, is a 110 kD protein which is expressed on monocytes/macrophages, endothelial cells, and at low levels on B cells and granulocytes. In association with a secretory molecule, MD2, TLR4 has been recognized as critical for host recognition of bacterial LPS. HTA125 antibody is useful for flow cytometric analysis and is able to block LPS-induced cytokine production.
Other Names:
Toll-like Receptor 4, TLR4, TLR-4, CD284
Structure:
Type I transmembrane protein, Toll-like receptor family/IL-1R superfamily, 110 kD
Function:
Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD284 (TLR4)
Human peripheral blood monocytes stained with HTA125 PE
LEAF™ Purified anti-human CD284 (TLR4)
Human peripheral blood monocytes stained with HTA125 PE
Biotin anti-human CD284 (TLR4)
Human peripheral blood monocytes stained with biotinylated HTA125, followed by Sav-PE
PE anti-human CD284 (TLR4)
Human peripheral blood monocytes stained with HTA125 PE
Brilliant Violet 421™ anti-human CD284 (TLR4)
Human peripheral blood monocytes were stained with CD284 (clone HTA125) Brilliant Violet 421™ (filled histogram) or mouse IgG2a Brilliant Violet 421™ isotype control (open histogram).
