Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:
WB1-Quality Tested IP1, Neutralization1* - *This application has been reported in the literature.
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, endothelial cell stimulation1 (enhanced proliferation, motility and migration, and induction of tube network). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 322203/322204).
Application References:
1. Yang, CR., et al., 2004. Cancer Res. 64:1122.
Description:
TL1A, also known as TNF ligand-related molecule 1, vascular endothelial growth inhibitor, and TNF ligand superfamily member 15, is a type II membrane protein with a predicted molecular weight of 28 kD. TL1A can also be found as a secreted protein as a result of proteolytic cleavage with a reported molecular weight of 32 kD. TL1A is a ligand for DR3 and DcR3 and is highly expressed in endothelial cells TL1A expression has also been observed in kidney, lung, prostate, placenta, and liver. Expression of TL1A is inducible with TNF and IL-1α (but not IFN-γ). TL1A acts to inhibits endothelial cell growth and has been reported to induce endothelial cell apoptosis and inhibit neovascularization in tumors. In lymphocytes, TL1A has been shown to enhance IL-2 receptor α and β expression and increase IL-2 induced proliferation and secretion of IFN-γ and GM-CSF. The 6E6 monoclonal antibody recognizes human TL1A and has been shown to be useful for immunoprecipitation and Western blot. In addition, the 6E6 antibody has been reported to enhance endothelial cell proliferation, motility and migration, and induce the formation of tube networks.
Member of the TNF ligand superfamily, type II membrane protein, can also exist as soluble form as a result of proteolytic cleavage. Predicted molecular weight approximately 28 kD; detected in culture supernatants as a 32 kD protein
Distribution:
Highly expressed in endothelial cells, also expressed in kidney, lung, prostate, placenta, and liver. Expression is inducible with TNF and IL-1α (but not IFN-γ)
Function:
Inhibits endothelial cell growth. Has been reported to induce endothelial cell apoptosis and inhibit neovascularization in tumors. In lymphocytes, can enhance IL-2 receptor α and ß expression and increase IL-2 induced proliferation and secret
Ligand Receptor:
Ligand for DR3 and DcR3
Antigen References:
1. Migone, T.S., et al., 2002. Immunity 16:479. 2. Tan, K.B., et al., 1997. Gene 204:35. 3. Zhai, Y., et al., 1999. FASEB J. 13:181. 4. Yue, T.-L., et al., 1999. J. Biol. Chem. 274:1479.
