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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, endothelial cell stimulation1 (enhanced proliferation, motility and migration, and induction of tube network). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 322203/322204).
Application References:
1. Yang CR, et al. 2004. Cancer Res. 64:1122. 2. Cunin P, et al. 2011. J. Immunol. 186:4175. PubMed.
Description:
TL1A, also known as TNF ligand-related molecule 1, vascular endothelial growth inhibitor, and TNF ligand superfamily member 15, is a type II membrane protein with a predicted molecular weight of 28 kD. TL1A can also be found as a secreted protein as a result of proteolytic cleavage, with a reported molecular weight of 32 kD. TL1A is a ligand for DR3 and DcR3 and is highly expressed in endothelial cells. TL1A expression has also been observed in kidney, lung, prostate, placenta, and liver. Expression of TL1A is inducible with TNF and IL-1α (but not IFN-γ). TL1A acts to inhibit endothelial cell growth and has been reported to induce endothelial cell apoptosis and inhibit neovascularization in tumors. In lymphocytes, TL1A has been shown to enhance IL-2 receptor α and β expression and increase IL-2 induced proliferation and secretion of IFN-γ and GM-CSF. The 6E6 monoclonal antibody recognizes human TL1A and has been shown to be useful for immunoprecipitation and Western blot. In addition, the 6E6 antibody has been reported to enhance endothelial cell proliferation, motility and migration, and induce the formation of tube networks.
Member of the TNF ligand superfamily, type II membrane protein, can also exist as soluble form as a result of proteolytic cleavage. Predicted molecular weight approximately 28 kD; detected in culture supernatants as a 32 kD protein
Distribution:
Highly expressed in endothelial cells, also expressed in kidney, lung, prostate, placenta, and liver. Expression is inducible with TNF and IL-1α (but not IFN-γ)
Function:
Inhibits endothelial cell growth. Has been reported to induce endothelial cell apoptosis and inhibit neovascularization in tumors. In lymphocytes, can enhance IL-2 receptor α and ß expression and increase IL-2 induced proliferation and secret
Ligand Receptor:
Ligand for DR3 and DcR3
Antigen References:
1. Migone TS, et al. 2002. Immunity 16:479. 2. Tan KB, et al. 1997. Gene 204:35. 3. Zhai Y, et al. 1999. FASEB J. 13:181. 4. Yue TL, et al. 1999. J. Biol. Chem. 274:1479.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
