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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 305614).
Note: EOS9.1 antibody (cat. No. 305703/305704) can induce apoptosis without cross-linking process.
Application References:
1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York. 2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York. 3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop) 4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC) 5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC) 6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed 7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. 9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Human peripheral blood lymphocytes stained with LEAF™ purified DX2, followed by anti-mouse IgGs FITC
CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.
Other Names:
Fas, APO-1, TNFRSF6
Structure:
TNFR superfamily, type I transmembrane protein, 45 kD
Distribution:
T and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes
Function:
Mediates apoptosis
Ligand Receptor:
Fas ligand (CD178)
Antigen References:
1. Krammer P, et al. 1994. Immunol. Rev. 142:175. 2. Nagata S, et al. 1995. Science 267:1449.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained with DX2 APC
Biotin anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained with biotinylated DX2, followed by Sav-PE
FITC anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained with DX2 FITC
LEAF™ Purified anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained with LEAF™ purified DX2, followed by anti-mouse IgGs FITC
PE anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained with DX2 PE
PE/Cy5 anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained with DX2 PE/CY5
Purified anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained
with DX2 PE/Cy5
Alexa Fluor® 488 anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained with DX2 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD95 (FAS)
Human peripheral blood lymphocytes stained with DX2 Alexa Fluor® 647
Brilliant Violet 421™ anti-human CD95 (FAS)
Human peripheral blood lymphocytes were stained with CD95 (clone DX2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ (open histogram).
Pacific Blue™ anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 Pacific blue™
PE/Cy7 anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with DX2 PE/CY7
