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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications: blocks CD62E binding to its ligand
Application References:
1. Tu L, et al. 1996. J. Immunol. 157:3995 2. Zhang Y and S. Neelamegham. 2002. Biophys. J. 83:1934
Description:
CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162).The HAE-1f antibody has been shown to recognize human CD62E and to be useful for flow cytometry and in vitro blocking.
Other Names:
E-selectin, ELAM-1, LECAM-1
Structure:
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution:
Highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression); can also be expressed on endothelial cells in the skin, bone marrow and placenta.
Function:
Involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand Receptor:
Siayl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported in interact with CD62E
Antigen References:
1. Collins T, et al. 1991. J. Biol. Chem. 266:2466 2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238 3. Berg EL, et al. 1991. J. Exp. Med. 174:1461. 4. Lawrence MB, et al. 1993. J. Immunol. 151:6338. 5. Walz G, et al. 1990. Science 250:1132
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
