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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro blocking1 of lymphoctes interaction and immunohistochemistry of acetone-fixed frozen sections1. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 322704).
Application References:
1. Personal communication.
Human peripheral blood lymphocytes stained with LEAF™ purified HCD54, followed by anti-mouse IgG FITC
CD54 is a 85-110 kD type I transmembrane protein also known as ICAM-1. It is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The expression of ICAM-1 on the cell surface is potently upregulated by activation; a soluble form of ICAM-1 can be released from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds to CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.
Other Names:
ICAM-1, Ly-47
Structure:
Type I membrane protein, Ig superfamily, 85-110 kD
Distribution:
Activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, dendritic cells. Cell surface expression upregulated upon activation; soluble ICAM-1 released from surface.
Function:
Cellular adhesion, involved in inflammation and immune reactions. CD54 expressed on endothelial cells in areas of inflammation plays a role in leukocyte extravasation. Major cellular receptor for rhinovirus.
Ligand Receptor:
CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) and has also been reported to bind hyaluronan and fibrinogen
Antigen References:
1. Voraberger G, et al. 1991 J. Immunol. 147:2777. 2. Staunton DE, et al. 1988. Cell 52:925. 3. Greve JM, et al. 1989. Cell 56:839.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD54
Human peripheral blood lymphocytes stained with purified HCD54, followed by anti-mouse IgG FITC
LEAF™ Purified anti-human CD54
Human peripheral blood lymphocytes stained with LEAF™ purified HCD54, followed by anti-mouse IgG FITC
Biotin anti-human CD54
Human peripheral blood lymphocytes stained with biotinylated HCD54, followed by Sav-PE
PE anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 PE
Alexa Fluor® 488 anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 Alexa Fluor® 488
APC anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 APC
Alexa Fluor® 647 anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 Alexa Fluor® 647
FITC anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 FITC.
