Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: inhibition of PGP activity and blocking of cytokine production1,3,7, immunoprecipitation1, immunohistochemistry of 4% paraformaldehyde-fixed frozen sections2 and immunohistochemistry of HOPE-fixed (HEPES-glutamic acid buffer-mediated organic solvent protection effect) paraffin-embedded sections4 , and Western blotting2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 309709).
Application References:
1. Flo, T., et al., 2000. J. Immunol. 164:2064. 2. Faure, E., et al., 2001. J. Immunol. 166:2018. 3. Sugawara, S., et al., 2001. Infect. Immun. 69:4951. 4. Droemann, D., et al., 2003. Histochem. Cell Biol. 119:103. 5. Chavakis, E., et al., 2007. Circ. Res. 100:204. 6. Fiala, M., et al., 2007. Proc. Natl. Acad. Sci. USA 10.1073/pnas.0701267104. 7. Goo, S.Y., et al., 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.
Human peripheral blood monocytes stained
with TL2.1 FITC
Description:
Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation.
Other Names:
Toll like receptor 2, TIL4, CD282
Structure:
IL-1R superfamily, TLR family, type 1 transmembrane protein, 90 kD
Distribution:
Monocytes, granulocytes, endothelial and epithelial cells
Function:
Activates innate host defense against bacterial pathogens
1. Lien, E., et al., 1999. J. Biol. Chem. 274:33419. 2. Lien, E. et al., 2001. J. Biol. Chem. 276:1873. 3. Sabroe, I., et al., 2002. J. Immunol. 168:4701
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