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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: blocking1-3 and immunohistochemical staining of acetone-fixed frozen or paraffin embedded sections1.
Application References:
1. Brown J, et al. 2003. J. Immunol. 170:1257. (FC IHC Block) 2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block) 3. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
Description:
CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1).This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance.CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-g production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation.Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types including T and B cells, antigen-presenting cells, and nonhematopoietic cells.
Other Names:
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)
Distribution:
T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells.
Ligand Receptor:
PD-1 (PDCD1)
Bioactivity/Activities:
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Its interaction with PDCD1 inhibit T-cell proliferation and cytokine production.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
