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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Chemotaxis of human CCR7-transfected BaF3 cells was performed in the presence of 15 ng/ml of recombinant CCL19. Addition of LEAF™ purified anti-human CCR7 (clone G043H7) inhibited migration of hCCR7 transfected BaF3 cells (ED 1.42 µg/ml).
CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity through their effects on interactions between T cells and dendritic cells. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B lymphocytes recirculate between B-cell-rich compartments (follicles or B zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.
Other Names:
BLR2, CDw197, EBI1, CMKBR7
Structure:
Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution:
T, B, NK, dendritic cells.
Function:
The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand Receptor:
CCL19 and CCL21.
Antigen References:
1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with purified anti-human CCR7/CD197 (clone G043H7) (filled histogram) or mouse IgG2a isotype control (open histogram), followed by goat anti-mouse IgG-PE.
Alexa Fluor® 488 anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 APC and CCR7/CD197 (clone G043H7) Alexa Fluor® 488 (top) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
Brilliant Violet 421™ anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7/CD197 (clone G043H7) Brilliant Violet™ 421 (top) or mouse IgG2a, κ Brilliant Violet™ 421 isotype control (bottom).
PE anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and CCR7/CD197 (clone G043H7) PE (top) or mouse IgG2a, κ PE isotype control (bottom).
APC/Cy7 anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) APC/Cy7 (top) or mouse IgG2a, κ APC/Cy7 isotype control (bottom).
Pacific Blue™ anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD197 (clone G043H7) Pacific Blue™ (top) or mouse IgG2a, κ Pacific Blue™ isotype control (bottom).
APC anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7 (clone G043H7) APC (top) or mouse IgG2a, κ APC isotype control (bottom).
FITC anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 APC and CD197 (clone G043H7) FITC (top) or mouse IgG2a, κ FITC isotype control (bottom).
Alexa Fluor® 647 anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) Alexa Fluor® 647 (top) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
PerCP/Cy5.5 anti-human CD197 (CCR7)
Human peripheral blood leucocytes were stained with CD3 FITC and CD197 (clone G043H7) PerCP/Cy5.5 (top) or mouse IgG2a, κ PerCP/Cy5.5 isotype control (bottom).
LEAF™ Purified anti-human CD197 (CCR7)
Chemotaxis of human CCR7-transfected BaF3 cells was performed in the presence of 15 ng/ml of recombinant CCL19. Addition of LEAF™ purified anti-human CCR7 (clone G043H7) inhibited migration of hCCR7 transfected BaF3 cells (ED 1.42 µg/ml).
Brilliant Violet 605™ anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7/CD197 (clone G043H7) Brilliant Violet 605™.
PE/Cy7 anti-human CD197 (CCR7)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) PE/Cy7 (top) or mouse IgG2a, κ PE/Cy7 isotype control (bottom).
