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0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The CD16 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 302014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302050) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Other Names:
FcγRIII
Structure:
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution:
NK cells, activated monocytes, macrophages, neutrophils
Function:
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand Receptor:
Aggregated IgG, IgG-antigen complex
Antigen References:
1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275. 2. Stroncek D, et al. 1991. Blood 77:1572. 3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 APC
Biotin anti-human CD16
Human peripheral blood lymphocytes stained with biotinylated 3G8 and Sav-PE
FITC anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 FITC
LEAF™ Purified anti-human CD16
Human peripheral blood lymphocytes stained with LEAF™ purified 3G8, followed by anti-mouse IgGs FITC
Brilliant Violet 711™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).
PE anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 PE
PE/Cy5 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 PE/Cy5
Purified anti-human CD16
Human peripheral blood lymphocytes stained with purified 3G8 and anti-mouse IgGs FITC
APC/Cy7 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 APC/Cy7
PE/Cy7 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 PE/Cy5
Alexa Fluor® 488 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 Alexa Fluor® 647
Pacific Blue™ anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 Pacific Blue™
Alexa Fluor® 700 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 Alexa fluor® 700
PerCP/Cy5.5 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 PerCP/Cy5.5
PerCP anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 PerCP
Brilliant Violet 421™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
Brilliant Violet 605™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).
Brilliant Violet 650™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 650™.
Brilliant Violet 785™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD56 APC and CD16 (clone 3G8) Brilliant Violet 785™ (top) or mouse IgG1, κ Brilliant Violet 785™ isotype control (bottom).
Brilliant Violet 510™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 510™.
Ultra-LEAF™ Purified anti-human CD16
Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified CD16 (clone 3G8), followed by anti-mouse IgG FITC.
