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LEAF™ Purified anti-human CD16 Antibody
Cat. # Size Price  

302013 50 µg $120.00
    
302014 500 µg $350.00
    
302033 1 mg $640.00
    
Product Details

Clone: 3G8 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
V NK80
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Immunogen: Human PMN cells
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The CD16 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
CyTOF® - Validated
IP, Stim, Block, IHC - Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
The 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 302014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302050) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:
  Publication Library
 Image 1
Human peripheral blood lymphocytes stained with LEAF™ purified 3G8, followed by anti-mouse IgGs FITC


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Antigen Details

Description: CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Other Names: FcγRIII
Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand Receptor: Aggregated IgG, IgG-antigen complex
Antigen
References:
1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
GeneID: 2214
UniProt: View information about CD16 on UniProt.org
Keywords: LEAF™ Purified anti-human CD16, 3G8, LEAF™, FcγRIII, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset, Flow Cytometry, Blocking, Costimulation, Immunohistochemistry, Immunoprecipitation, Biofunctional Assay, Immunology, Antibodies
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Related Protocols

Cell Surface Immunofluorescence Staining Protocol
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Biofunctional Antibodies - LEAF™ and Ultra-LEAF™
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Version: 2 Revision Date: 2014-05-23
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 APC

  • Biotin anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated 3G8 and Sav-PE

  • FITC anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 FITC

  • LEAF™ Purified anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified 3G8, followed by anti-mouse IgGs FITC

  • Brilliant Violet 711™ anti-human CD16
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).

  • PE anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 PE

  • PE/Cy5 anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 PE/Cy5

  • Purified anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified 3G8 and anti-mouse IgGs FITC

  • APC/Cy7 anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 APC/Cy7

  • PE/Cy7 anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 PE/Cy5

  • Alexa Fluor® 488 anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 Alexa Fluor® 647

  • Pacific Blue™ anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 Pacific Blue™

  • Alexa Fluor® 700 anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 Alexa fluor® 700

  • PerCP/Cy5.5 anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 PerCP/Cy5.5

  • PerCP anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 3G8 PerCP

  • Brilliant Violet 421™ anti-human CD16
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

  • Brilliant Violet 570™ anti-human CD16
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).

  • Brilliant Violet 605™ anti-human CD16
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).

  • Brilliant Violet 650™ anti-human CD16
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 650™.

  • Brilliant Violet 785™ anti-human CD16
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD56 APC and CD16 (clone 3G8) Brilliant Violet 785™ (top) or mouse IgG1, κ Brilliant Violet 785™ isotype control (bottom).





  • Brilliant Violet 510™ anti-human CD16
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 510™.

  • Ultra-LEAF™ Purified anti-human CD16
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified CD16 (clone 3G8), followed by anti-mouse IgG FITC.

  • Purified anti-human CD16 (MaxPar® Ready)
    Human PBMCs stained with 152Sm-anti-CD13

    Human PBMCs stained with 152Sm-anti-CD13 (WM15) and 148Nd-anti-CD16 (3G8). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

  • PE/Dazzle™ 594 anti-human CD16
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).

  • Purified anti-CD16
    CD16 (clone 3G8) and isotype

    CD16 (clone 3G8) and isotype control staining of whole human blood analyzed by flow cytometry. A goat anti-mouse-PE conjugate was used as secondary antibody.

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