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LEAF™ Purified anti-human CD151 (PETA-3) Antibody
LEAF™ Purified anti-human CD151 (PETA-3) Antibody
350403 50 µg $100.00     
350404 500 µg $320.00     
Cat. No. 350403 is scheduled for discontinuation. Please use the availability checker to determine current availability.

Product Details

 
Clone: 50-6 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
Reactivity: Human
Immunogen: Human epidermoid carcinoma cell line HEp-3
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:

FC - Quality tested
Block, ICC, WB - Reported in literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Application
Notes:

Additional reported applications (for the relevant formats) include: in vivo inhibits metastasis of HEp-3 cells1, Western blot1, immunocytochemical staining1 of HEp-3 cells, and CD151-transfected COS cells fixed with 0.25% glutaraldehyde.

Application
References:

  Publication Library
LEAF™ Purified anti-human CD151 (PETA-3) Antibody 50-6 Image 1
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC


Compare all formats



Description:

CD151, also known as PETA-3, is a 29 kD protein member of the tetraspanin family (TM4SF). As other members in the family, PETA-3 is composed of two extracellular loops (one larger than the other), four transmembrane domains and two cytoplasmic tails. Also, as other members in the family, CD151 is involved in cell adhesion, migration, and tumor cell metastasis. PETA-3 is expressed by many different cell types such as immature haemopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells and vascular endothelium. In the cell surface PETA-3 associates with CD9, CD181, and the integrins α3β1, α5β1 and α6β4.

Other Names: Platelet-endothelial tetraspan antigen 3, GP27, Membrane glycoprotein SFA-1, Tetraspanin-24, Tspan-24
Structure: Member of the tetraspanin family (TM4SF), 29 kD protein, consist of two extracellular loop, four transmembrane domains and two cytoplasmic tails.
Distribution: Immature haemopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium
Function: Cell adhesion, migration
Interaction: CD9, CD181, integrins α3β1, α5β1 and α6β4
Antigen
References:

1. Zöller M. 2009. Nat. Rev. Cancer 9:40.
2. Yañez-Mó M, et al. 2008. Blood 112:3217.
3. Nishiuchi R, et al. 2005. Proc. Natl. Acad. Sci. USA 102:1939.

GeneID: 977
Latest Publications: View the latest CD151 articles on HighwirePress.com
UniProt: View information about CD151 on UniProt.org
Keywords: LEAF™ Purified anti-human CD151 (PETA-3), 50-6, LEAF™, Platelet-endothelial tetraspan antigen 3, GP27, Membrane glycoprotein SFA-1, Tetraspanin-24, Tspan-24 , Human, Flow Cytometry, Blocking, Immunocytochemistry, Western Blotting, Biofunctional Assay, Immunology, Antibodies
Related Products

DescriptionCloneApplications
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
LEAF™ Purified Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICFC, WB, IP, ICC, IF, FA


Other Formats

DescriptionCloneApplications
APC anti-human CD151 (PETA-3)50-6FC
Purified anti-human CD151 (PETA-3)50-6FC, ICC, WB

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD151 (PETA-3)
    Human peripheral blood platelets stained

    Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC

  • APC anti-human CD151 (PETA-3)
    Human peripheral blood platelets stained

    Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC

  • LEAF™ Purified anti-human CD151 (PETA-3)
    Human peripheral blood platelets stained

    Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC

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