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0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vivo inhibits metastasis of HEp-3 cells1, Western blot1, immunocytochemical staining1 of HEp-3 cells, and CD151-transfected COS cells fixed with 0.25% glutaraldehyde.
CD151, also known as PETA-3, is a 29 kD protein member of the tetraspanin family (TM4SF). As other members in the family, PETA-3 is composed of two extracellular loops (one larger than the other), four transmembrane domains and two cytoplasmic tails. Also, as other members in the family, CD151 is involved in cell adhesion, migration, and tumor cell metastasis. PETA-3 is expressed by many different cell types such as immature haemopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells and vascular endothelium. In the cell surface PETA-3 associates with CD9, CD181, and the integrins α3β1, α5β1 and α6β4.
1. Zöller M. 2009. Nat. Rev. Cancer 9:40. 2. Yañez-Mó M, et al. 2008. Blood 112:3217. 3. Nishiuchi R, et al. 2005. Proc. Natl. Acad. Sci. USA 102:1939.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD151 (PETA-3)
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
APC anti-human CD151 (PETA-3)
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
LEAF™ Purified anti-human CD151 (PETA-3)
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
