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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vivo inhibits metastasis of HEp-3 cells1, Western blot1, immunocytochemical staining1 of HEp-3 cells, and CD151-transfected COS cells fixed with 0.25% glutaraldehyde.
Application References:
1. Testa JE, et al. 1999. Cancer Res. 59:3812. (Block ICC WB)
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
CD151, also known as PETA-3, is a 29 kD protein member of the tetraspanin family (TM4SF). As other members in the family, PETA-3 is composed of two extracellular loops (one larger than the other), four transmembrane domains and two cytoplasmic tails. Also, as other members in the family, CD151 is involved in cell adhesion, migration, and tumor cell metastasis. PETA-3 is expressed by many different cell types such as immature haemopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells and vascular endothelium. In the cell surface PETA-3 associates with CD9, CD181, and the integrins α3β1, α5β1 and α6β4.
1. Zöller M. 2009. Nat. Rev. Cancer 9:40. 2. Yañez-Mó M, et al. 2008. Blood 112:3217. 3. Nishiuchi R, et al. 2005. Proc. Natl. Acad. Sci. USA 102:1939.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD151 (PETA-3)
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
APC anti-human CD151 (PETA-3)
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
LEAF™ Purified anti-human CD151 (PETA-3)
Human peripheral blood platelets stained with anti-human purified CD151 (clone 50-6) conjugated with APC
