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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 301708).
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Saiki I, et al. 1993. Int J Cancer. 54:137. (Block) 3. Rosenzwajg M, et al. 2000. Blood 95:453. (Block) 4. Kawase M, et al. 2008. J Virol. 83:712. (Block) PubMed
Human peripheral blood granulocytes stained with LEAF™ purified WM15, followed by anti-mouse IgGs FITC
CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. This zinc metallopeptidase as a homodimer is expressed on granulocytes, myeloid progenitors, endothelial cells, epithelial cells and subset of granular lymphoid cells. It is not expressed on platelets or erythrocytes. CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. CD13 serves as the cellular receptor for Coronavirus.
Other Names:
Aminopeptidase N, APN, gp150
Structure:
Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD13
Human peripheral blood lymphocytes, monocytes and granulocytes stained with WM15 APC
PE anti-human CD13
Human peripheral blood granulocytes stained with WM15 PE
Purified anti-human CD13
Human peripheral blood granulocytes stained with purified WM15, followed by anti-mouse IgGs FITC
LEAF™ Purified anti-human CD13
Human peripheral blood granulocytes stained with LEAF™ purified WM15, followed by anti-mouse IgGs FITC
APC/Cy7 anti-human CD13
Human peripheral blood granulocytes stained with WM15 APC/Cy7 and overlayed with isotype control
PE/Cy7 anti-human CD13
Human peripheral blood granulocytes were stained with CD13 (clone WM15) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).
