Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:
FC - Quality tested. IHC(Frozen)4*, Blocking5,6,9* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining4 of acetone-fixed frozen tissue sections, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 301616).
Application References:
1. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp, W., et al., 1989. Leucocyte Typing IV, Oxford University Press. New York. 3. McMichael, A., et al., Eds. 1987. Leucocyte Typing III, Oxford University Press. New York. 4. Vainer, B., et al., 2000. Am. J. Sur. Patho. 24:1115. 5. Ottonello, L., et al., 1999. Blood 93:3505. 6. Metelitsa, L. S., et al., 2002. Blood 99:4166. 7. Sadhu, C., et al., 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. 8. Ihanus E., et al., 2007. Blood. 109:802-810. 9. Sadu, C., et al.2008. J Immunoassay Immunochem. 29:42.
Human peripheral blood granulocytes stained with 3.9 PE
Description:
CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b. Clone 3.9 antibody preferentially binds the activated form of CD11c, is specific for I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent.
Other Names:
Integrin αX subunit, CR4, p150
Structure:
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Ligand Receptor:
CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Antigen References:
1. Petty, H. 1996. Immunol. Today 17:209. 2. Springer, T. 1994. Cell 76:301. 3. Ihanus E., et al., 2007. Blood. 109:802-810.
