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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Blocking the binding of SCF. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 323404).
Human erythroleukemic cell line TF-1 stained with LEAF™ purified A3C6E2, followed by biotinylated anti-mouse IgG and Sav-PE
CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemic cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117, and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. The A3C6E2 antibody potently blocks the binding of SCF.
Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decre
Ligand Receptor:
c-Kit ligand
Modification:
Multiple phosphorylation sites
Antigen References:
1. Giebel LB, et al. 1992. Oncogene 7:2207. 2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD117 (c-kit)
Human erythroleukemic cell line TF-1 stained with purified A3C6E2, followed by biotinylated anti-mouse IgG and Sav-PE
LEAF™ Purified anti-human CD117 (c-kit)
Human erythroleukemic cell line TF-1 stained with LEAF™ purified A3C6E2, followed by biotinylated anti-mouse IgG and Sav-PE
Biotin anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with biotinylated A3C6, followed by Sav-PE
PE anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE
PE/Cy5 anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE/Cy5
PE/Cy7 anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE/Cy7
