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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: blocking5,6 of immunoglobulin binding to FcγRI, and immunohistochemical staining of acetone-fixed frozen tissue sections.
Application References:
1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York. 2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 4. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. 5. Holl V, et al. 2004. J. Immunol. 173:6274. 6. Hober D, et al. 2002. J. Gen. Virol. 83:2169. 7. Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006 8. Van Tits L, et al.2005. Arteisocler Thromb Vasc Biol. 25:717. PubMed 9. Bruhns P, et al. 2008. BloodPubMed 10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 11. Carter DL, et al. 1999. Cytometry 37:41. (FC)
Human peripheral blood monocytes stained with purified 10.1, followed by anti-mouse IgG FITC
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD64
Human peripheral blood monocytes stained
with 10.1 FITC
FITC anti-human CD64
Human peripheral blood monocytes stained
with 10.1 FITC
PE anti-human CD64
Human peripheral blood monocytes stained with 10.1 PE
Purified anti-human CD64
Human peripheral blood monocytes stained with purified 10.1, followed by anti-mouse IgG FITC
Alexa Fluor® 488 anti-human CD64
Human peripheral blood lymphocytes, monocytes, and granulocytes stained with 10.1 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD64
Human peripheral blood monocytes stained with10.1 Alexa Fluor® 647
APC anti-human CD64
Human peripheral blood monocytes stained with 10.1 APC
LEAF™ Purified anti-human CD64
Human peripheral blood monocytes stained with purified 10.1, followed by anti-mouse IgG FITC
Pacific Blue™ anti-human CD64
Human peripheral blood monocytes stained with 10.1 Pacific Blue™
