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High molecular weight glycoproteins from rat thymocytes
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The OX-8 antibody has been reported to partially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation. Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation3, in vivo and in vitro blocking of T cell responses3, 4, macrophage stimulation5, and Western blotting3,6.
Application References:
1. Barclay AN. 1981. Immunology 42:593. (IHC) 2. Wallgren AC, et al. 1995. Transplantation 60:594. (IHC) 3. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP, WB) 4. Mason DW, et al. 1983. Immunol. Rev. 74:57. 5. Hirji N, et al. 1997.J. Immunol.158:1833. (FA) 6. Mitnacht R,, et al.1998. J. Immunol.160:700. (WB)
Description:
CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Other Names:
CD8a, T8, Leu-8, Ly-2, Lyt2
Structure:
Ig superfamily, approximately 32 kD
Distribution:
Thymocytes, NK cells, most CD8+ cells, intestinal intraepithelial lymphocytes, activated CD4+ cells, macrophages
Function:
T cell activation, co-receptor for T cell activation
Ligand Receptor:
MHC class I molecule
Antigen References:
1. Johnson P, et al. 1985. EMBO J. 4:2539. 2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
