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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications include: immunoprecipitation1, in vitro activation, in vitro inhibition1 of binding of VCAM-1 and mast cells to fibronectin , and in vivo inhibition of VLA-4 functions1. The LEAF™Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 200106).
Application References:
1. Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP, Block)
LOU rat splenocytes stained with LEAF™ purified MRα4-1, followed by anti-mouse IgG FITC
CD49d is a 150 kD glycoprotein, also known as integrin α4 or VLA-4 α chain. It is expressed as a heterodimer with either of two β chains, β1 or β7. The α4β1 integrin (also known as VLA-4, CD49d/CD29) is expressed on peripheral T and B lymphocytes, thymocytes, and monocytes, while the α4β7 integrin (also known as LPAM-1) is expressed on peripheral lymphocytes. The α4β1 and α4β7 integrins mediate cell-cell and cell-matrix interactions by interacting with the ligands CD106 (VCAM-1), MAdCAM-1, and fibronectin. Soluble MRα4-1 antibody can partially inhibit in vitro binding of VCAM-1 and mast cells to fibronectin and inhibit mast cell degranulation. Immobilized MRα4-1 antibody can enhance mast cell degranulation in vitro.
Other Names:
Integrin α4 chain, α4 integrin, VLA-4 α chain
Structure:
Integrin α chain, 150 kD
Distribution:
α4/β1- peripheral T and B lymphocytes, thymocytes, and monocytes; α4/β7- peripheral lymphocytes
Function:
Adhesion
Ligand Receptor:
CD106 (VCAM-1), MAdCAM-1, fibronectin
Antigen References:
1. Yasuda M, et al. 1995. Int. Immunol. 7:251. 2. Springer TA. 1994. Cell 76:301. 3. Issekutz TB, et al. 1991. J. Immunol. 147:4178. 4. Issekutz TB, et al. 1991. J. Immunol. 147:109. 5. Yang H, et al. 1995. Transplantation 60:71. 6. Palecanda A, et al. 1997. J. Immunol. 158:2904.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-rat CD49d
Lou rat blood lymphocytes stained
with MRα4-1 FITC
LEAF™ Purified anti-rat CD49d
LOU rat splenocytes stained with LEAF™ purified MRα4-1, followed by anti-mouse IgG FITC
