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F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.
Application References:
1. Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ IHC IP) 2. Nicholls MR, et al. 1993. Transplantation 55:459. (Block) 3. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF) 4. Baba T, et al. 2006. Blood 107:2004. (IHC) 5. Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
LOU rat splenocytes stained with purified 1F4, followed by anti-mouse Ig FITC
CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.
Other Names:
T cell antigen receptor complex, T3
Structure:
Ig superfamily, approximately 20-25 kD
Distribution:
Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
Function:
Antigen recognition, TCR signal transduction, T cell activation
Ligand Receptor:
Peptide antigen/MHC complex
Antigen References:
1. Tanaka T, et al. 1989 J. Immunol. 142:2791. 2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-rat CD3
LOU rat splenocytes stained with purified 1F4, followed by anti-mouse Ig FITC
FITC anti-rat CD3
LOU rat splenocytes stained with 1F4 FITC
Alexa Fluor® 488 anti-rat CD3
LOU rat splenocytes stained with 1F4 Alexa Fluor® 488
Alexa Fluor® 647 anti-rat CD3
LOU rat splenocytes stained with 1F4 Alexa Fluor® 647
LEAF™ Purified anti-rat CD3
LOU rat splenocytes stained with purified 1F4, followed by anti-mouse Ig FITC
