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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) VEGF-A antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The VEGF-A antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 2-6 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Neutralization: The LEAF™ Purified antibody is recommended for neutralization of mouse VEGF-A bioactivity in vivo3 and in vitro3. Western Blotting: It is suggested to use 0.5-2 µg/ml for WB3. Immunohistochemistry: Clone 2G11-2A05 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 µg/ml. ELISA or ELISPOT: The LEAF™ Purified 2G11-2A05 antibody is useful in a mouse VEGF-A ELISA or ELISPOT assay. Functional Assay: The LEAF™ Purified 2G11-2A05 antibody is useful in a mouse VEGF-A functional assay3.
It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Reinders MEJ, et al. 2003. J. Clin. Invest. 112:1655. 2. Wuest TR, et al. 2009. J. Exp Med. 207;101. (Neut) PubMed 3. Basu A, et al. 2008. Cancer Res. 68:5689. (Neut WB FA) PubMed 4. Kumar V, et al. 2010. Blood 115:4725. PubMed
Western blot analysis of recombinant mouse VEGF protein using anti-VEGF, clone 2F11-2A05.
VEGF-A, also known as VEGF (vascular endothelial growth factor), is the founding member of the family including VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides with a highly conserved receptor-binding cystine-knot structure similar to that of the platelet-derived growth factors.VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.VEGF-A is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. Oxygen tension is a key physiological regulator of VEGF-A gene expression.
Other Names:
Vascular endothelial growth factor (VEGF)
Structure:
cystine-knot growth factors
Distribution:
aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells
Function:
angiogenesis
Regulation:
HIF-1, hypoxiaresponsiveness
Cellular Targets:
endothelial cells
Receptors:
VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1)
Antigen References:
1. Contreras A, et al. 2007. Am. J. Transpl. 7:155. 2. Lambrechts D and Carmeliet P. 2007. Biochim. Biophys. Acta. 1762:1109. 3. Tong JP and Yao YF. 2006. Clin. Biochem. 39:267. 4. Reinders MEJ, et al. 2003. J. Clin. Invest. 112:1655 5. Ferrara N, et al. 2003. Nat. Med. 9:669. 6. Shibuya M. 2001. Cell Struct. Funct. 26:25. 7. Senger DR, et al. 1983. Science. 219:983.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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LEAF™ Purified anti-mouse VEGF-A
Western blot analysis of recombinant mouse VEGF protein using anti-VEGF, clone 2F11-2A05.
