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Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
In addition to flow cytometric uses, the MTS510 antibody has also been used to block LPS-induced stimulation and immunoprecipitation.
Application References:
1. Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block) 2. Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC) 3. Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed 4. Kanada S, et al. 2011. Blood 117:2211. PubMed
Mouse TLR4/MD2 transfected cells were stained with LEAF™ purified CD284 (clone MTS510) (filled histogram), or LEAF™ purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells and T cells. TLR4 has been recognized as critical for host recognition of bacterial LPS in conjunction with MD2.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse TLR4 (CD284)/MD2 Complex
Mouse TLR4/MD2 transfected cells were stained with purified CD284 (clone MTS510) (filled histogram), or purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
Mouse TLR4/MD2 transfected cells were stained with LEAF™ purified CD284 (clone MTS510) (filled histogram), or LEAF™ purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
Biotin anti-mouse TLR4 (CD284)/MD2 Complex
Thioglycollate-elicited mouse peritoneal macrophages were stained with biotinylated CD284 (clone MTS510) (filled histogram), or biotinylated rat IgG2a, κ (open histogram), followed by Sav- PE.
PE anti-mouse TLR4 (CD284)/MD2 Complex
Thioglycollate-elicited mouse peritoneal macrophages were stained with CD284 (clone MTS510) PE (solid line), or rat IgG2a, κ PE (broken line).
PE/Cy7 anti-mouse TLR4 (CD284)/MD2 Complex
Mouse TLR4/MD2/CD14 transfected cells were stained with CD284 (clone MTS510) PE/Cy7 (red histogram), or rat IgG2a, κ PE/Cy7 (blue histogram).
