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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
Moriyama Y, et al. 2008. Int J Immunology 20:763
Mouse NOTCH-2 transfected cells stained with LEAF™ purified HMN2-35, followed by anti-Armenian hamster IgG PE
The Notch receptors are highly conserved from invertebrates to mammals.While Notch1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences.The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus.This translocation triggers a series of signaling process.Notch 3 is primarily expressed in adult arterial smooth muscle cells.Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.
Other Names:
Neurogenic locus notch homolog protein 2, Notch-2
Structure:
Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution:
Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. In spleen, Notch 2 is reported to be expressed primarily on B cells.
Function:
Regulate broad spectrum of various cell lineage commitment process, including myogenesis, neurogenesis, gliogenesis and lymphocyte development. Mutation of Notch2 can cause a rare genetic disorder, Alagille syndrome, with multiple defects in liver, heart and other systems.
Ligand Receptor:
Jag1,2; Delta 1,4
Antigen References:
1. Ehebauer ME, et al. 2006. Biochem J 392:13 2. Shimizu K, et al. 2000. Mol Cell Biology 20:18 3. Tanigaki K, et al. 2007. Nature Immunol 8:451 4. Kraman M, et al. 2005. FASEB J. 19:1311
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Notch 2
FN2/CHO cells stained with purified HMN2-35, followed by anti-Armenian hamster IgG PE
LEAF™ Purified anti-mouse Notch 2
Mouse NOTCH-2 transfected cells stained with LEAF™ purified HMN2-35, followed by anti-Armenian hamster IgG PE
PE anti-mouse Notch 2
Mouse Notch-2 transfected cells stained with HMN2-35 PE
APC anti-mouse Notch 2
Mouse Notch 2 transfected cells stained with HMN2-35 APC
