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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Moriyama Y, et al. 2008. Int. Immunol. 20:763 (FC)
FJ2/CHO transfected cells stained with LEAF™ purified HMJ2-1, followed by anti-Armenian hamster IgG PE
The Notch receptors and their ligands are highly conserved from invertebrates to mammals. The Jagged-2 is one of the several Notch ligands identified. The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus. This translocation triggers a series of signaling process. Jagged-2 is reported to be essential for the regulation of spermatogenesis.
Other Names:
HJ2, Jagged2, Jagged-2 precursor, JAG2
Structure:
A single pass transmembrane protein consisting of a large extracellular N-terminal which includes the DSL domain, followed by a tandem array of EGF-like repeats (ELRs) and cysteine-rich domain (CRD). After a single transmembrane domain (TM) is a short in
Distribution:
Testicular tissues and spermatids
Function:
The Notch receptors and their ligands are highly conserved signaling molecules throughout development. They regulate cell fate decision for various cell development, including myogenesis, neurogenesis, and lymphocyte development. There is growing evidence that signals transmitted through different Notch ligands can differentially affect the target cells. Notch1/Jagged-2 signaling system plays an important role for male germ cells to differentiate.
Ligand Receptor:
Notch receptors
Cellular Targets:
Skeletrophin binds the intracellular regions of Jagged-2, but not those of Delta-1,3,4, or Jagged-1.
Antigen References:
1. Ehebauer MT, et al. 2006. Biochem J 392:13 2. Shimizu K, et al. 2000. Mol Cell Biology 20:18 3. Hellstrom M, et al. 2007. Nature 445:776 4. Luo B, et al. 1997. Mol Cell Biol. 17:6057
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Jagged 2
FJ2/CHO transfectant cell line stained with HMJ2-1 purified, followed by anti-hamster IgG PE
LEAF™ Purified anti-mouse Jagged 2
FJ2/CHO transfected cells stained with LEAF™ purified HMJ2-1, followed by anti-Armenian hamster IgG PE
PE anti-mouse Jagged 2
FJ2/CHO transfected cells stained with HMJ2-1 PE
Alexa Fluor® 647 anti-mouse Jagged 2
FJ2/CHO transfectant cell line stained with HMJ2-1 Alexa Fluor® 647
