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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) IL-4 antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The IL-4 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this IL-4 antibody is quality control tested by ELISA assay. For ELISPOT applications, a concentration range of 2-6 µg/ml is recommended. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-4 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA1,2,10,13 or ELISPOT5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 563201) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization1-2,9,12: The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108). Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections6,7, and immunocytochemistry. Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.
Application References:
1. Shirai A, et al. 1994. Cytokine 6:329. (ELISA, Neut) 2. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA, Neut) 3. Assenmacher M, et al. 1994. Eur. J. Immunol. 24:1097. 4. Openshaw P, et al. 1995. J. Exp. Med. 182:1357. 5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISA Capture) 6. Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC) 7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC) 8. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC) 9. Hara M, et al. 2001. J. Immunol. 166:3789. (Neut) 10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA) 11. Lawson BR, et al. 2007. J. Immunol. 178:5366. 12. Wang W, et al. 2007. J. Immunol. 178:4885. (Neut) 13. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed 14. Ohnmacht C, et al. 2008. Blood 113:2816. PubMed 15. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.
Other Names:
Interleukin-4, Ia inducing factor (IaIF), B cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Structure:
Cytokine; 15-19 kD (Mammalian)
Regulation:
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Cellular Sources:
Mast cells, T cells, bone marrow stromal cells
Cellular Targets:
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors:
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Bioactivity/Activities:
Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294. 3. Dullens H, et al. 1991. In vivo 5:567. 4. Paul W. 1991. Blood 77:1859.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse IL-4
PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized C57BL/6 CD4-positive cells were surface stained with CD3 PE and then intracellularly stained with IL-4 (11B11) APC (top) or rat IgG1, κ APC isotype control (bottom).
LEAF™ Purified anti-mouse IL-4
PE anti-mouse IL-4
PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized C57BL/6 CD4-positive cells were surface stained with CD3 APC and then intracellularly stained with IL-4 (11B11) PE (top) or Rat IgG1, κ PE isotype control (bottom).
Purified anti-mouse IL-4
Alexa Fluor® 488 anti-mouse IL-4
PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized C57BL/6 CD4-positive T cells were surface stained with CD3 APC and then intracellularly stained with IL-4 (11B11) Alexa Fluor® 488 (top) or rat IgG1, κ Alexa Fluor® 488 isotype control (bottom).
Alexa Fluor® 647 anti-mouse IL-4
PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized C57BL/6 CD4-positive cells were surface stained with CD3 PE and then intracellularly stained with IL-4 (11B11) Alexa Fluor® 647 (top) or rat IgG1, κ Alexa Fluor® 647 isotype control (bottom).
PE/Cy7 anti-mouse IL-4
PMA+ionomycin-stimulated (in presence of Brefeldin A for 6 hours) Th2-polarized C57BL/6 mouse CD4+ T cells were surface stained with CD3 and then intracelluarly stained with IL-4 (clone 11B11) PE/Cy7 (top) or rat IgG1, κ PE/Cy7 isotype control (bottom).
Brilliant Violet 421™ anti-mouse IL-4
PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized C57BL/6 T cells were surface stained with CD4 APC and then intracellularly stained with IL-4 ( clone 11B11) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).
